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Overview

  • Product nameAnti-CCR5 antibodySee all CCR5 primary antibodies ...
  • Description
    Rabbit polyclonal to CCR5
  • Tested applicationsWB, ICC/IF more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: N terminus of human CCR5.

  • Positive controlTHP 1 or CEM x 174 cell lysate

Applications

Our Abpromise guarantee covers the use of ab21653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
ICC/IF
  • Application notesICC/IF: Use at an assay dependent dilution.
    WB: 1/1000 - 1/2000. Detects a band of approximately 41 kDa.
    Note: a 41 kDa immunoreactive protein was observed in supernatants. This band is abolished by immunizing peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionReceptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
    • Tissue specificityHighly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.
    • Involvement in diseaseGenetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
    • Post-translational
      modifications      Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
      O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
      Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
      Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES.
    • Cellular localizationCell membrane.

      • Target information above from: UniProt accession P51681 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          AM4 7 antibodyC C chemokine receptor type 5 antibodyC C CKR 5 antibody
          C-C chemokine receptor type 5 antibodyC-C CKR-5 antibodyC-C motif chemokine receptor 5 A159A antibodyCC Chemokine Receptor 5 antibodyCC Chemokine Receptor Type 5 antibodyCC CKR 5 antibodyCC-CKR-5 antibodyCCCKR 5 antibodyCCCKR5 antibodyCCR 5 antibodyCCR-5 antibodyCCR5 antibodyCCR5 chemokine (C C motif) receptor 5 antibodyCCR5_HUMAN antibodyCD 195 antibodyCD195 antibodyCD195 Antigen antibodyChemokine C C motif receptor 5 antibodyChemokine receptor CCR5 antibodyCHEMR13 antibodyCKR 5 antibodyCKR5 antibodyCMKBR 5 antibodyCMKBR5 antibodyFLJ78003 antibodyHIV 1 Fusion Coreceptor antibodyHIV-1 fusion coreceptor antibodyHIV1 fusion coreceptor antibodyIDDM22 antibodyMIP-1 alpha receptor antibody
        see all

      Anti-CCR5 antibody images

      • Western blot - CCR5 antibody (ab21653)
        Western blot - CCR5 antibody (ab21653)
        Western blot analysis of CCR5 in CEM x 174 supernatants (A, B) and precipitants (C, D) with anti CCR5 (NT) at 1/1000 dilution.

      References for Anti-CCR5 antibody (ab21653)

      This product has been referenced in:
      • Karnoub AE  et al. Mesenchymal stem cells within tumour stroma promote breast cancer metastasis. Nature 449:557-63 (2007). ICC/IF ; Human . Read more (PubMed: 17914389) »

      See 1 Publication for this product

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