Anti-CCR5 antibody [mAbcam 64620] (ab64620)

Overview

• Product nameAnti-CCR5 antibody [mAbcam 64620]See all CCR5 primary antibodies ...
• Description
  Mouse monoclonal [mAbcam 64620] to CCR5
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Chimpanzee, Baboon, Macaque Monkey, Orangutan
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CCR5.

  Read Abcam's proprietary immunogen policy

• Positive controlThis antibody gave a positive signal in human spleen tissue lysate and in the following whole cell lysates: THP1; Jurkat; K562.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone numbermAbcam 64620
• MyelomaSp2/0-Ag14
• IsotypeIgG
• Research Areas
  • Cancer
  • Signal transduction
  • G protein signaling
  • GPCR

  • Signal Transduction
  • Signaling Pathway
  • G Protein Signaling
  • GPCR

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Leukocyte recruitment
  • Chemokines

  • Microbiology
  • Interspecies Interaction
  • Host Virus Interaction

  • Immunology
  • Immune System Diseases
  • Antiviral Signaling
  • HIV-related

  • Immunology
  • Innate Immunity
  • Macrophage / Inflamm.

  • Immunology
  • Innate Immunity
  • Chemokines
  • Beta Chemokine Rec. (CCR)

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Other

Applications

Target

• FunctionReceptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
• Tissue specificityHighly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.
• Involvement in diseaseGenetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
• Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
• Post-translational
  modificationsSulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
  O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
  Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
  Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES.
• Cellular localizationCell membrane.

Target information above from: UniProt accession P51681 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 450128 Chimpanzee
  • Entrez Gene: 1234 Human
  • Entrez Gene: 1234 Human
  • Omim: 601373 Human
  • SwissProt: P56440 Chimpanzee
  • SwissProt: P51681 Human
  • SwissProt: P51681 Human
  • Unigene: 450802 Human

• Alternative names
  AM4 7 antibodyC C chemokine receptor type 5 antibodyC C CKR 5 antibody

  C-C chemokine receptor type 5 antibodyC-C CKR-5 antibodyC-C motif chemokine receptor 5 A159A antibodyCC Chemokine Receptor 5 antibodyCC Chemokine Receptor Type 5 antibodyCC CKR 5 antibodyCC-CKR-5 antibodyCCCKR 5 antibodyCCCKR5 antibodyCCR 5 antibodyCCR-5 antibodyCCR5 antibodyCCR5 chemokine (C C motif) receptor 5 antibodyCCR5_HUMAN antibodyCD 195 antibodyCD195 antibodyCD195 Antigen antibodyChemokine C C motif receptor 5 antibodyChemokine receptor CCR5 antibodyCHEMR13 antibodyCKR 5 antibodyCKR5 antibodyCMKBR 5 antibodyCMKBR5 antibodyFLJ78003 antibodyHIV 1 Fusion Coreceptor antibodyHIV-1 fusion coreceptor antibodyHIV1 fusion coreceptor antibodyIDDM22 antibodyMIP-1 alpha receptor antibody

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Anti-CCR5 antibody [mAbcam 64620] images

• 

  Western blot - CCR5 antibody (ab64620)
  All lanes : Anti-CCR5 antibody [mAbcam 64620] (ab64620) at 10 µg/ml
  
  Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
  Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
  Lane 4 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 41 kDa
  Observed band size : 41 kDa
  Additional bands at : 51 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 1 minute