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All lanes : Anti-CCR5 (phospho S349) antibody (ab55180) at 1/500 dilution
Lane 1 : extracts from RAW264.7 cells
treated with PMA (125ng/ml, 30mins).
Lane 2 : extracts from RAW264.7 cells
treated with PMA (125ng/ml, 30mins), and treated with the immunising phosphopeptide.
Predicted band size : 41 kDa
Observed band size : 51 kDa (why is the actual band size different from the predicted?)
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