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Products:Immunology >> Innate Immunity >> Chemokines >> Beta Chemokine Rec. (CCR)
Overview
Product name
Anti-CCR6 antibody
See all CCR6 products (5) ...
Description
Rabbit polyclonal to CCR6
Specificity
No cross reactivity with other proteins.
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to a sequence at the N terminal of human CCR6.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide, 0.01% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Immunology >> Innate Immunity >> Chemokines >> Beta Chemokine Rec. (CCR)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab78429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.75 µg/mlPredicted molecular weight: 42 kDa.
IHC-P: Use a concentration of 0.5 - 1 µg/ml.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Function
Receptor for a C-C type chemokine. Binds to MIP-3-alpha/LARC and subsequently transduces a signal by increasing the intracellular calcium ions level.
Tissue specificity
Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP51684
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BN 1 antibody
- BN1 antibody
- C C chemokine receptor type 6 antibody
see all
Database links
- Entrez Gene: 1235 Human
- Entrez Gene: 12458 Mouse
- Entrez Gene: 308163 Rat
- Omim: 601835 Human
- SwissProt: P51684 Human
- SwissProt: O54689 Mouse
- SwissProt: Q5BK58 Rat
- Unigene: 46468 Human
see all
Anti-CCR6 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CCR6 antibody (ab78429)
ab78429 at 0.5ug/ml staining CCR6 in human large intestine tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.
Western blot - CCR6 antibody (ab78429)
All lanes : Anti-CCR6 antibody (ab78429) at 0.75 µg/ml
Lane 1 : Tissue lysate prepared from Rat Thymus
Lane 2 : Whole cell lysate prepared from Human MCF7 cells
Lane 3 : Whole cell lysate prepared from Hela cells
Lane 4 : Cell lysate prepared from smmc cells
Lysates/proteins at 50 µg per lane.
Secondary
HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution
Predicted band size : 42 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
References for Anti-CCR6 antibody (ab78429)
ab78429 has not yet been referenced specifically in any publications.
Publishing research using ab78429? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"