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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CCR6 antibody (ab78429)
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Western blot - CCR6 antibody (ab78429)
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Product Name
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CCR6 antibody
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See all CCR6 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CCR6
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Immunogen
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A synthetic peptide corresponding to a sequence at the N terminal of human CCR6.
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Reacts with
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Hu, Rat
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Predicted to react
(species key)
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Ms
(Due to sequence homology)
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Specificity
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No cross reactivity with other proteins.
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 0.75 µg/ml. Predicted molecular weight: 42 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell membrane; Multipass membrane protein.
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Research areas
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Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Immunology >> Innate Immunity >> Chemokines >> Beta Chemokine Rec. (CCR)
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Relevance
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CCR6 is a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein coupled receptors. It is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP 3 alpha). This receptor has been shown to be important for B lineage maturation and antigen driven B cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses.
CCR6 has been reported to be expressed in intestine, colon, liver, lymph node, spleen, lung, blood, umbilical cord, placenta, skin, and pancreas. It has also been reported to be expressed in lymphocytes, T cells, and B cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05mg Sodium Azide, 0.05mg Thimerosal
Constituents: 5mg BSA, 0.9mg Sodium chloride, 0.2mg Dibasic Sodium phosphate Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CCR6 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CCR6 antibody images, references, products related to ab78429 and other tools.
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CCR6 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CCR6 antibody (ab78429)
ab78429 at 0.5ug/ml staining CCR6 in human large intestine tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
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Western blot - CCR6 antibody (ab78429)
All lanes : CCR6 antibody (ab78429) at 0.75 µg/ml
Lane 1 : Tissue lysate prepared from Rat Thymus
Lane 2 : Whole cell lysate prepared from Human MCF7 cells
Lane 3 : Whole cell lysate prepared from Hela cells
Lane 4 : Cell lysate prepared from smmc cells
Predicted band size : 42 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to CCR6
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CCR6 antibody - more information
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Customer reviews (feedback) regarding CCR6 antibody |
Customer FAQs regarding CCR6 antibody |
Protocols for CCR6 antibody |
Price and availability of products related to CCR6 antibody |
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CCR6 antibody - related products:
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CCR6 antibody - other tools:
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Contact Abcam with a Technical Enquiry about CCR6 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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