Anti-CCR9 antibody [7E7] (ab52590)
Key features and details
- Rat monoclonal [7E7] to CCR9
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
-
Product name
Anti-CCR9 antibody [7E7]
See all CCR9 primary antibodies -
Description
Rat monoclonal [7E7] to CCR9 -
Host species
Rat -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide:
PTDFTSPIPNMADDYGSEST
, corresponding to N terminal amino acids 3-22 of Mouse CCR9 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 5% Fetal calf serum -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
7E7 -
Myeloma
P3x63-Ag8.653 -
Isotype
IgG2b -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/20.
|
|
Flow Cyt |
1/20.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
---|
ICC/IF
1/20. |
Flow Cyt
1/20. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. -
Tissue specificity
Highly expressed in the thymus and low in lymph nodes and spleen. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 10803 Human
- Entrez Gene: 12769 Mouse
- Omim: 604738 Human
- SwissProt: P51686 Human
- SwissProt: Q9WUT7 Mouse
- Unigene: 225946 Human
- Unigene: 440604 Mouse
- Unigene: 442383 Mouse
-
Alternative names
- C C chemokine receptor D6 antibody
- C C chemokine receptor type 9 antibody
- C C CKR 9 antibody
see all
Images
-
ab52590 staining CCR9 in Mouse brain tissue sections by Immunocytochemistry/ Immunofluorescence.
Red = CCR9
Green = IgA
Blue = CD3
-
Overlay histogram showing K562 cells stained with ab52590 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52590, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab52590 has been referenced in 1 publication.
- Pabst O et al. Chemokine receptor CCR9 contributes to the localization of plasma cells to the small intestine. J Exp Med 199:411-6 (2004). PubMed: 14744993