Overview

  • Product nameAnti-CCR9 antibody [CW-1.2] (FITC)
    See all CCR9 primary antibodies
  • Description
    Mouse monoclonal [CW-1.2] to CCR9 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsFlow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Monkey
  • Immunogen

    WT Mouse thymocytes (Eur J Immunol. 2006 Jan;36(1):73-81)

  • Positive control
    • Mouse thymocytes and splenocytes (BALB/c splenocytes)

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 1X PBS,10% HPCD pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • Clone numberCW-1.2
  • IsotypeIgG2a
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab95670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.125-0.25µg for 105-8 cells. A final volume of 100 µL is recommended for staining the cell sample.

Target

  • RelevanceThe protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. This gene is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members; however, its specific function remains unknown. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to chromosome 3p21.3, a region that includes a cluster of chemokine receptor genes. Two alternatively spliced transcript variants have been described.
  • Cellular localizationCell membrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • C C chemokine receptor D6 antibody
    • C C chemokine receptor type 9 antibody
    • C C CKR 9 antibody
    • CC Chemokine receptor CCR10 antibody
    • CC CKR 9 antibody
    • CCBP2 antibody
    • CCCKR9 antibody
    • CCR 9 antibody
    • CCR9 antibody
    • CDw199 antibody
    • CDw199 antigen antibody
    • Chemokine (C C motif) receptor 9 antibody
    • Chemokine (C C motif) receptor 9, isoform CRA_a antibody
    • Chemokine (C-C motif) receptor 9 isoform A antibody
    • Chemokine (C-C motif) receptor 9 isoform B antibody
    • Chemokine C C motif receptor 9 antibody
    • Chemokine C C receptor 10 antibody
    • Chemokine CC Motif Receptor 9 antibody
    • Chemokine receptor CCR 9 antibody
    • Chemokine receptor CCR9 antibody
    • Chemokine-binding protein D6 antibody
    • Cmkbr10 antibody
    • CMKBR9 antibody
    • G protein coupled receptor 28 antibody
    • GPR 28 antibody
    • GPR 9 6 antibody
    • GPR28 antibody
    • GPR96 antibody
    • OTTHUMP00000164653 antibody
    • OTTHUMP00000164654 antibody
    see all

Anti-CCR9 antibody [CW-1.2] (FITC) images

  • Flow cytometry staining of BALB/c splenocytes with 0.125 µg of Mouse IgG2a ? Isotype Control FITC (open histogram) or 0.125 µg of ab95670 (filled histogram). Cells in the lymphocyte gate were used for analysis and gated on either CD4+ (left) or CD8+ (right) events.
  • ab95670 staining CCR9 in Rat splenocytes by Flow Cytometry. Rat spleen was homogenized and red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.

    See Abreview

References for Anti-CCR9 antibody [CW-1.2] (FITC) (ab95670)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Flow Cytometry
Permeabilization No
Sample Cynomolgus Monkey Cell (blood)
Specification blood
Gating Strategy alive cells
Preparation Cell harvesting/tissue preparation method: red blood cell lysis
Sample buffer: PBS/BSA 0.5%
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Abcam user community

Verified customer

Submitted Dec 26 2013

Application Flow Cytometry
Permeabilization No
Sample Human Cell (PBMC)
Specification PBMC
Gating Strategy alive lymphocytes
Preparation Cell harvesting/tissue preparation method: ficoll
Sample buffer: PBS/BSA 0.5%
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Abcam user community

Verified customer

Submitted Dec 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Dog Cell (from whole blood)
Specification from whole blood
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive cells
Username

Abcam user community

Verified customer

Submitted Nov 12 2012

Application Flow Cytometry
Sample Cynomolgus Monkey Cell (Whole blood)
Specification Whole blood
Preparation Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Apr 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: spleen was mashed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization No
Gating Strategy PBS/BSA 1%/Azide 0.01%
Username

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Verified customer

Submitted Apr 23 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (PBMC)
Specification PBMC
Preparation Cell harvesting/tissue preparation method: from buffy coat after ficoll preparation
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation none
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Apr 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (Whole blood)
Specification Whole blood
Preparation Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Apr 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"