Anti-CCR9 antibody [CW-1.2] (FITC) (ab95670)
- Product nameAnti-CCR9 antibody [CW-1.2] (FITC)See all CCR9 primary antibodies ...
- DescriptionMouse monoclonal [CW-1.2] to CCR9 (FITC)
- Tested applicationsFlow Cyt more details
- Species reactivityReacts with: Mouse, Rat, Dog, Monkey
WT Mouse thymocytes (Eur J Immunol. 2006 Jan;36(1):73-81)
- Positive controlMouse thymocytes and splenocytes (BALB/c splenocytes)
- Storage instructionsStore at +4°C in the dark. Do not freeze.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 1X PBS,10% HPCD pH 7.2
- Concentration information loading...
- PurityProtein G purified
- Clonality Monoclonal
- Clone numberCW-1.2
- Research Areas
Our Abpromise guarantee covers the use of ab95670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Flow Cyt: Use 0.125-0.25µg for 105-8 cells. A final volume of 100 µL is recommended for staining the cell sample.|
- RelevanceThe protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. This gene is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members; however, its specific function remains unknown. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to chromosome 3p21.3, a region that includes a cluster of chemokine receptor genes. Two alternatively spliced transcript variants have been described.
- Cellular localizationCell membrane; Multi-pass membrane protein.
- C C chemokine receptor D6 antibodyC C chemokine receptor type 9 antibodyC C CKR 9 antibody
- CC Chemokine receptor CCR10 antibodyCC CKR 9 antibodyCCBP2 antibodyCCCKR9 antibodyCCR 9 antibodyCCR9 antibodyCDw199 antibodyCDw199 antigen antibodyChemokine (C C motif) receptor 9 antibodyChemokine (C C motif) receptor 9, isoform CRA_a antibodyChemokine (C-C motif) receptor 9 isoform A antibodyChemokine (C-C motif) receptor 9 isoform B antibodyChemokine C C motif receptor 9 antibodyChemokine C C receptor 10 antibodyChemokine CC Motif Receptor 9 antibodyChemokine receptor CCR 9 antibodyChemokine receptor CCR9 antibodyChemokine-binding protein D6 antibodyCmkbr10 antibodyCMKBR9 antibodyG protein coupled receptor 28 antibodyGPR 28 antibodyGPR 9 6 antibodyGPR28 antibodyGPR96 antibodyOTTHUMP00000164653 antibodyOTTHUMP00000164654 antibody
Anti-CCR9 antibody [CW-1.2] (FITC) images
Flow cytometry staining of BALB/c splenocytes with 0.125 µg of Mouse IgG2a ? Isotype Control FITC (open histogram) or 0.125 µg of ab95670 (filled histogram). Cells in the lymphocyte gate were used for analysis and gated on either CD4+ (left) or CD8+ (right) events.
ab95670 staining CCR9 in Rat splenocytes by Flow Cytometry. Rat spleen was homogenized and red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.