The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.125-0.25µg for 105-8 cells. A final volume of 100 µL is recommended for staining the cell sample.
ab91362-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
RelevanceThe protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. This gene is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members; however, its specific function remains unknown. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to chromosome 3p21.3, a region that includes a cluster of chemokine receptor genes. Two alternatively spliced transcript variants have been described.
Flow cytometry staining of BALB/c splenocytes with 0.125 µg of Mouse IgG2a ? Isotype Control FITC (open histogram) or 0.125 µg of ab95670 (filled histogram). Cells in the lymphocyte gate were used for analysis and gated on either CD4+ (left) or CD8+ (right) events.
Flow Cytometry - Anti-CCR9 antibody [CW-1.2] (FITC) (ab95670)This image is courtesy of an anonymous Abreview
ab95670 staining CCR9 in Rat splenocytes by Flow Cytometry. Rat spleen was homogenized and red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.