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CCRF-CEM (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14642)

Q&A (2)

Overview

  • Product nameCCRF-CEM (Human T cell lymphoblast-like cell line) Nuclear Lysate
  • General notesWe recommend aliquoting the extracts into single use fractions and then storing.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research Areas
  • BackgroundThe direct isolation and propagation of a continuous culture of human lymphoblasts (CCRF-CEM) (also called CEM) from the peripheral blood of a patient with acute lymphoblastic leukemia has permitted extensive sequential cytogenetic analysis of these cells during continuous propagation in culture.

    • Applications

  • Application notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References for CCRF-CEM (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14642)

    ab14642 has not yet been referenced specifically in any publications.

    Publishing research using ab14642 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab14642

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    Do you have karyotype info on the CCRFCEM line that these nuclear lysates came from? I have been working with CEM-C7 and CEM C1 cells and would like to utilitze your lysate as a positive control, but want to make sure the karyotype is the same.

    Thank you for your enquiry. This extract was prepared from the parental cell line CCRF-CEM (ATCC number CCL-119) Karyotype: modal number = 47; range = 41 to 95. Fifty karyotypes showed no consistent loss or gain of particular chromosomes. Twen...

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    Ab14642 is listed as being nuclear lysate, but on the datasheet under purity it states whole cell lysate. Please clarify.

    Thank you for your phone call. I have confirmed that ab14642 is CCRF-CEM nuclear extract, and have updated the online datasheet. We unfortunately do not currently carry CCRF-CEM whole cell lysate. If you have any additional questions, please contact us ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"