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Products:Immunology >> Adaptive Immunity >> B Cells >> CD
Overview
Product name
Anti-CD10 + CD19 antibody [FR4D11/F3 + CB19] (Dual color: FITC + PE)
See all CD10 + CD19 products (2) ...
Description
Mouse monoclonal [FR4D11/F3 + CB19] to CD10 + CD19 (Dual color: FITC + PE)
Conjugation
Dual color: FITC + PE
Tested applications
Flow Cytmore details
Cross reactivity
Reacts with
Human
General notes
This product is a mixture of two clones:
CD10 = Clone:FR4D11/F3, Isotype:IgG1, Label:FITC
CD19 = Clone:CB19, Isotype:IgG1, Label:PE
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark. Do not freeze.
Storage buffer
PBS with 0.5% BSA and 0.1% sodium azide
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
FR4D11/F3 + CB19
Myeloma
unknown
Light chain type
unknown
Research areas
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Immunology >> Adaptive Immunity >> T Cells >> CD
Immunology >> Adaptive Immunity >> B Cells >> CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt
Application notes
Characterization of leukaemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient.
Childhood ALL prognosis (patients CD10+ have a better prognosis than CD10-)
CD10(FITC) / CD19 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
10µl ofCD10(FITC) / CD19 (PE) is sufficient for labelling 1x106 cells.
Target
Relevance
The CD10 antigen (Mw = 100kDa) or "Common Acute Lymphoblastic Leukaemia Antigen" (CALLA) is expressed on the majority of B lymphoid leukaemias. It is also present on a wide variety of normal and neoplastic cell types including renal epithelium, fibroblasts, granulocytes, pre B and pre T cells, and some neural cells and neoplastic cell types including lymphomas and melanomas. It is a cell surface enzyme with endopeptidase activity. CD19 antigen (MW = 95kDa) is present on 10-15% of human peripheral blood lymphocytes and approximately 60% of splenic lymphocytes. It is not expressed on T lymphocytes, granulocytes, activated T cells or monocytes. Moreover, CD19 is present on principal acute and chronic B cell leukemias and lymphomas.
Cellular localization
CD10: Membrane; single pass type II membrane protein. CD19: Membrane; single pass type I membrane protein.
Alternative names
- Atriopeptidase antibody
- B lymphocyte antigen CD19 antibody
- CD10 antibody
see all
Database links
- Entrez Gene: 4311 Human
- Entrez Gene: 930 Human
- Omim: 107265 Human
- Omim: 120520 Human
- SwissProt: P08473 Human
- SwissProt: P15391 Human
- Unigene: 307734 Human
References for Anti-CD10 + CD19 antibody [FR4D11/F3 + CB19] (Dual color: FITC + PE) (ab1237)
ab1237 has not yet been referenced specifically in any publications.
Publishing research using ab1237? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"