![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD10 antibody [56C6] (ab951) image](#CD10-Primary-antibodies-ab951-3.jpg)
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Products:Immunology >> Adaptive Immunity >> B Cells >> CD
Overview
Product name
Anti-CD10 antibody [56C6]
See all CD10 products (33) ...
Description
Mouse monoclonal [56C6] to CD10
Tested applications
WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Prokaryotic recombinant fusion protein corresponding to the external of the CD10 glycoprotein
Positive control
Tonsil, kidney or lymphoblastic leukemia.
General notes
This antibody works well when used in combination with ab972 - Antigen retrieval solution, heat mediated high pH 9.5.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, Carrier protein, Renoir Red Diluent, pH 7.3
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
56C6
Myeloma
unknown
Isotype
IgG1
Light chain type
unknown
Research areas
Cancer >> Tumor immunology >> CD markers
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative
Immunology >> Cell Type Markers >> CD >> Non-lineage
Immunology >> Adaptive Immunity >> B Cells >> CD
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD10 antibody [56C6] (ab951)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD10 antibody [56C6] (ab951) image](/ps/datasheet/images/0/ab951/CD10-Primary-antibodies-ab951-3.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Predicted molecular weight: 85 kDa.
IHC-P: 1/25 - 1/50.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.(For an ABC system, incubate for 30-60 minutes at RT. With high pH retrieval solutions, some tissues may require an avidin-biotin block. Overstaining with hematoxylin can mask staining, thus reducing overall contrast and making interpretation difficult. It is therefore recommended that a light hematoxylin stain be applied.Recently, CD10 has been used in a panel for mantle cell lymphoma with cyclin D1(+), CD43 (+), CD5 (+), IgM (+), CD23 (-) and CD10 (-).)
IHC-Fr: 1/25 - 1/50.(For an ABC system, incubate for 30-60 minutes at RT. )
Target
Function
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Sequence similarities
Belongs to the peptidase M13 family.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP08473
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Atriopeptidase antibody
- CALLA antibody
- CD 10 antibody
see all
Database links
- Entrez Gene: 4311 Human
- Entrez Gene: 17380 Mouse
- Omim: 120520 Human
- SwissProt: P08473 Human
- SwissProt: Q61391 Mouse
- Unigene: 307734 Human
- Unigene: 296022 Mouse
Anti-CD10 antibody [56C6] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD10 antibody [56C6] (ab951)
ab951 at 1/20 dilution staining CD10 in MDA-MB-231 human breast cancer cells grown in mouse by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed prior to blocking in 10% casein for 30 minutes at 22°C and then incubated with ab951 for 17 hours at 4°C. A rabbit polyclonal to mouse Ig, diluted 1/200 was used as the secondary antibody.
This image is courtesy of an anonymous Abreview.
References for Anti-CD10 antibody [56C6] (ab951)
This product has been referenced in:
- Spencer Bet al. Peripheral delivery of a CNS targeted, metalo-protease reduces aß toxicity in a mouse model of Alzheimer's disease. PLoS One 6:e16575 (2011). WB; Mouse.Read more (PubMed: 21304989) »
- Niikura Tet al. A humanin derivative reduces amyloid Beta accumulation and ameliorates memory deficit in triple transgenic mice. PLoS One 6:e16259 (2011). IHC-Fr; Mouse.Read more (PubMed: 21264226) »
See all 8 publications for this product
Publishing research using ab951? Please let us know so that we can cite the reference in this datasheet
![CD10 antibody [56C6] for WB in Human (951)](/ps/datasheet/images/0/ab951/CD10-Primary-antibodies-ab951-1.JPG)
![CD10 antibody [56C6] for IHC-P in Human (951)](/ps/datasheet/images/0/ab951/CD10-Primary-antibodies-ab951-2.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"