CD10 antibody [56C6] (ab951)

Product Name 

CD10 antibody [56C6]

Product type 

Primary antibodies

Description 

Mouse monoclonal [56C6] to CD10

Immunogen 

Prokaryotic recombinant fusion protein corresponding to the external of the CD10 glycoprotein

Reacts with 
(species key)

Hu

Tested applications 
(see key)

IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
WB: 1/1000. Predicted molecular weight: 85 kDa.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
For an ABC system, dilute 1:25-50 and incubate for 30-60 minutes at RT.
For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval before commencing with IHC.
With high pH retrieval solutions, some tissues may require an avidin-biotin block.
Overstaining with hematoxylin can mask staining, thus reducing overall contrast and making interpretation difficult.
It is therefore recommended that a light hematoxylin stain be applied.
Recently, CD10 has been used in a panel for mantle cell lymphoma with cyclin D1(+), CD43 (+), CD5 (+), IgM (+), CD23 (-) and CD10 (-).

Positive control 
(see definition)

Tonsil, kidney or lymphoblastic leukemia.

Cellular localization 

Type II membrane protein.

Research areas 

Cancer >> Tumor immunology >> CD markers
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative
Immunology >> Cell Type Markers >> CD >> Non-lineage
Immunology >> Adaptive Immunity >> B Cells >> CD

Relevance 

CD10, also known as the Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

56C6

Isotype 

IgG1

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: PBS, Carrier protein, Renoir Red Diluent, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C.

Notes 

This antibody works well when used in combination with ab972 - Antigen retrieval solution, heat mediated high pH 9.5.

References for CD10 antibody [56C6] (ab951)

  This product has been used in: (two most recent references)

Gutierrez-Gonzalez L et al. Analysis of the clonal architecture of the human small intestinal epithelium establishes a common stem cell for all lineages and reveals a mechanism for the fixation and spread of mutations. J Pathol 217:489-96 (2009). IHC-P; Human. PubMed: 19156773

Rockenstein E et al. High beta-secretase activity elicits neurodegeneration in transgenic mice despite reductions in amyloid-beta levels: implications for the treatment of Alzheimer disease. J Biol Chem 280:32957-67 (2005). PubMed: 16027115

See all 4 publication references for this product.


If you publish research using ab951 please let us know so that we can cite the reference on this datasheet.

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