![Western blot - CD10 antibody [EPR2997] (ab79423) image](#CD10-Primary-antibodies-ab79423-1.jpg)
 image](#CD10-Primary-antibodies-ab79423-2.jpg)
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Products:Immunology >> Adaptive Immunity >> B Cells >> CD
Overview
Product name
Anti-CD10 antibody [EPR2997]
See all CD10 products (33) ...
Description
Rabbit monoclonal [EPR2997] to CD10
Tested applications
WB, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues on the N terminal of Human CD10.
Positive control
Jurkat and LnCap lysates.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR2997
Isotype
IgG
Research areas
Cancer >> Tumor immunology >> CD markers
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative
Immunology >> Cell Type Markers >> CD >> Non-lineage
Immunology >> Adaptive Immunity >> B Cells >> CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab79423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000 - 1/10000.Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa).
Flow Cyt: 1/100 - 1/150.
Application notes
Is unsuitable for or IP.
Target
Function
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Sequence similarities
Belongs to the peptidase M13 family.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP08473
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Atriopeptidase antibody
- CALLA antibody
- CD 10 antibody
see all
Database links
- Entrez Gene: 4311 Human
- Entrez Gene: 17380 Mouse
- Entrez Gene: 24590 Rat
- Omim: 120520 Human
- SwissProt: P08473 Human
- SwissProt: Q61391 Mouse
- SwissProt: P07861 Rat
- Unigene: 307734 Human
see all
Anti-CD10 antibody [EPR2997] images:
Western blot - CD10 antibody [EPR2997] (ab79423)
![Western blot - CD10 antibody [EPR2997] (ab79423)](/ps/datasheet/images/79/ab79423/CD10-Primary-antibodies-ab79423-1.jpg)
All lanes : Anti-CD10 antibody [EPR2997] (ab79423) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : LnCap cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 86 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Flow Cytometry-CD10 antibody [EPR2997](ab79423)
](/ps/datasheet/images/79/ab79423/CD10-Primary-antibodies-ab79423-2.jpg)
Overlay histogram showing Jurkat cells stained with ab79423 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79423, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
References for Anti-CD10 antibody [EPR2997] (ab79423)
ab79423 has not yet been referenced specifically in any publications.
Publishing research using ab79423? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"