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Overview

  • Product nameAnti-CD10 antibody [MEM-78] (FITC)See all CD10 primary antibodies ...
  • Description
    Mouse monoclonal [MEM-78] to CD10 (FITC)
  • ConjugationFITC
  • Conjugation notesAb28096 is free of unconjugated FITC.
  • SpecificityAb28096 recognises CD10.
  • Tested applicationsFlow Cyt more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue / cell preparation (Human): Pre B cell line NALM-6.

Properties

Applications

Our Abpromise guarantee covers the use of ab28096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Flow Cyt: Use 20µl for 106 cells. or 100 µl of whole blood.

Target

  • FunctionThermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
  • Sequence similaritiesBelongs to the peptidase M13 family.
  • Cellular localizationCell membrane.

    • Target information above from: UniProt accession P08473 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Atriopeptidase antibodyCALLA antibodyCD 10 antibody
        CD10 antibodyCD10 antigen antibodyCommon acute lymphocytic leukemia antigen antibodyDKFZp686O16152 antibodyEC 3.4.24.11 antibodyEnkephalinase antibodyEPN antibodygp100 antibodyMembrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibodyMembrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibodyMembrane metallo endopeptidase antibodyMembrane metallo endopeptidase variant 1 antibodyMembrane metallo endopeptidase variant 2 antibodyMembrane metalloendopeptidase antibodyMembrane metalloendopeptidase neutral endopeptidase enkephalinase antibodyMembrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibodyMembrane metalloendopeptidase variant 1 antibodyMembrane metalloendopeptidase variant 2 antibodyMGC126681 antibodyMGC126707 antibodyMME antibodyNEP antibodyNEP_HUMAN antibodyNeprilysin antibodyNeutral endopeptidase 24.11 antibodyNeutral endopeptidase antibodySFE antibodySkin fibroblast elastase antibody
      see all

    References for Anti-CD10 antibody [MEM-78] (FITC) (ab28096)

    This product has been referenced in:
    • Horejsí V  et al. Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha) 34:23-34 (1988). Read more (PubMed: 2968928) »

    See 1 Publication for this product

    Publishing research using ab28096 ? Please let us know so that we can cite the reference in this datasheet

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    Do I need to use a permeabilisation step for flow with this antibody and if so, what should I use?

    Thank you for your enquiry. The originator of this antibody tests it in flow cytometry using NALM-6 cell line and it works well. Permeabilization is not a necessary step in the flow cytometry protocol for this antibody. I hope this information hel...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"