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Flow Cytometry / FACS - CD10 antibody [SN5c] (ab34199)
![Flow Cytometry / FACS - CD10 antibody [SN5c] (ab34199)](/ps/datasheet/images/ab34199-1.JPG)
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Product Name
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CD10 antibody [SN5c] - Azide free
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See all CD10 antibodies (36)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [SN5c] to CD10 - Azide free
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Immunogen
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Partially purified cell membrane antigens from fresh leukemia cells.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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Flow Cyt, IHC-Fr, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use 10µl to label 106 cells in 100µl.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 85 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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human peripheral blood granulocytes
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Cellular localization
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Type II membrane protein.
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Research areas
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Cancer >> Tumor immunology >> CD markers
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative
Immunology >> Cell Type Markers >> CD >> Non-lineage
Immunology >> Adaptive Immunity >> B Cells >> CD
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Relevance
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CD10, also known as the Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.
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CD10 antibody [SN5c] - Azide free (ab34199) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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SN5c
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Myeloma
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P3-NS1/1-Ag4-1
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: PBS, pH 7.4
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Purification notes
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Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD10 antibody [SN5c] - Azide free is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD10 antibody [SN5c] - Azide free images, references, products related to ab34199 and other tools.
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CD10 antibody [SN5c] - Azide free images:
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 Flow Cytometry / FACS - CD10 antibody [SN5c] (ab34199)
ab34199, Using Neat dilution, Staining of human peripheral blood granulocytes by Flow Cytometry.
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References for CD10 antibody [SN5c] - Azide free (ab34199)
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This product has been used in: (two most recent references)
Biddle WC et al. In vitro and in vivo cytotoxic activity of anti-human leukemia monoclonal antibodies SN5c and SN6 daunorubicin conjugates. Leuk Res 13:699-707 (1989). PubMed: 2529399
Matsuzaki H et al. Unique epitopes of common acute lymphoblastic leukemia antigen detected by new monoclonal antibodies. Cancer Res 47:2160-6 (1987). PubMed: 2435407
If you publish research using ab34199 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to CD10
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CD10 antibody [SN5c] - Azide free - more information
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Customer reviews (feedback) regarding CD10 antibody [SN5c] - Azide free |
Customer FAQs regarding CD10 antibody [SN5c] - Azide free |
Protocols for CD10 antibody [SN5c] - Azide free |
Price and availability of products related to CD10 antibody [SN5c] - Azide free |
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CD10 antibody [SN5c] - Azide free - related products:
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CD10 antibody [SN5c] - Azide free - other tools:
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Contact Abcam with a Technical Enquiry about CD10 antibody [SN5c] - Azide free |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Flow Cytometry / FACS - CD10 antibody [SN5c] (ab34199)](/index.html?datasheet=34199#ab34199-1.JPG)
