![Flow Cytometry / FACS - CD10 antibody [SN5c] (ab34199) image](#ab34199-1.JPG)
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Products:Immunology >> Adaptive Immunity >> B Cells >> CD
Overview
Product name
Anti-CD10 antibody [SN5c] - Azide free
See all CD10 products (33) ...
Description
Mouse monoclonal [SN5c] to CD10 - Azide free
Tested applications
WB, IP, IHC-Fr, Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
Partially purified cell membrane antigens from fresh leukemia cells.
Positive control
human peripheral blood granulocytes
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Clonality
Monoclonal
Clone number
SN5c
Myeloma
P3-NS1/1-Ag4-1
Isotype
IgG1
Research areas
Cancer >> Tumor immunology >> CD markers
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative
Immunology >> Cell Type Markers >> CD >> Non-lineage
Immunology >> Adaptive Immunity >> B Cells >> CD
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Flow Cytometry / FACS - CD10 antibody [SN5c] (ab34199)
![Flow Cytometry / FACS - CD10 antibody [SN5c] (ab34199) image](/ps/datasheet/Images/34/ab34199/ab34199-1.JPG)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab34199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 85 kDa.
IP: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
Flow Cyt: Use 10µl for 106 cells.
Target
Function
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Sequence similarities
Belongs to the peptidase M13 family.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP08473
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Atriopeptidase antibody
- CALLA antibody
- CALLA antibody
see all
Database links
- Entrez Gene: 4311 Human
- Omim: 120520 Human
- SwissProt: P08473 Human
- Unigene: 307734 Human
Anti-CD10 antibody [SN5c] - Azide free images:
Flow Cytometry / FACS - CD10 antibody [SN5c] (ab34199)
ab34199, Using Neat dilution, Staining of human peripheral blood granulocytes by Flow Cytometry.
References for Anti-CD10 antibody [SN5c] - Azide free (ab34199)
This product has been referenced in:
- Biddle WCet al. In vitro and in vivo cytotoxic activity of anti-human leukemia monoclonal antibodies SN5c and SN6 daunorubicin conjugates. Leuk Res 13:699-707 (1989).Read more (PubMed: 2529399) »
- Matsuzaki Het al. Unique epitopes of common acute lymphoblastic leukemia antigen detected by new monoclonal antibodies. Cancer Res 47:2160-6 (1987).Read more (PubMed: 2435407) »
See all 2 publications for this product
Publishing research using ab34199? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"