Anti-CD105 antibody [MEM-229] (ab69772)

Overview

  • Product nameAnti-CD105 antibody [MEM-229]See all CD105 primary antibodies ...
  • Description
    Mouse monoclonal [MEM-229] to CD105
  • Tested applicationsICC, IHC-Fr, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA.

  • Positive control
    • Kg1 human acute myelogenous leukemia cell line. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab69772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-Fr 1/200. Fix with Acetone.
Flow Cyt Use a concentration of 10 µg/ml.
WB Use at an assay dependent dilution. Use under non reducing condition. Predicted molecular weight: 71 kDa.

Target

  • FunctionMajor glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • Tissue specificityEndoglin is restricted to endothelial cells in all tissues except bone marrow.
  • Involvement in diseaseDefects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI528660 antibody
    • AI662476 antibody
    • CD 105 antibody
    • CD105 antibody
    • CD105 antigen antibody
    • EGLN_HUMAN antibody
    • END antibody
    • Endoglin antibody
    • ENG antibody
    • FLJ41744 antibody
    • HHT1 antibody
    • ORW antibody
    • ORW1 antibody
    • Osler Rendu Weber syndrome 1 antibody
    • RP11 228B15.2 antibody
    • S-endoglin antibody
    • SN6 antibody
    see all

Anti-CD105 antibody [MEM-229] images

  • ab69772 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab69772 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CD105 antibody [MEM-229] (ab69772)

ab69772 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. I recommend the following isotype controls for each of these antibodies: ab5694 - Rabbit IgG ab27478 Click here (or use the following: http://www.abcam.c...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"