Anti-CD105 antibody [MEM-229] (ab69772)
- Product nameAnti-CD105 antibody [MEM-229]See all CD105 primary antibodies ...
- DescriptionMouse monoclonal [MEM-229] to CD105
- Tested applicationsICC, IHC-Fr, Flow Cyt, WB more details
- Species reactivityReacts with: Human, Pig
Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA.
- Positive controlKg1 human acute myelogenous leukemia cell line. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
- Concentration information loading...
- PurityProtein A purified
- Purification notesab69772 was purified from ascites. Purity is greater than 95% by SDS-PAGE.
- Clonality Monoclonal
- Clone numberMEM-229
- Research Areas
Our Abpromise guarantee covers the use of ab69772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||ICC: Use at an assay dependent concentration.|
|IHC-Fr||IHC-Fr: 1/200. Fix with Acetone.|
|Flow Cyt||Flow Cyt: Use a concentration of 10 µg/ml.|
|WB||WB: Use at an assay dependent dilution. Use under non reducing condition. Predicted molecular weight: 71 kDa.|
- FunctionMajor glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
- Tissue specificityEndoglin is restricted to endothelial cells in all tissues except bone marrow.
- Involvement in diseaseDefects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
- Cellular localizationMembrane.
- CD 105 antibodyCD105 antibodyCD105 antigen antibody
- EGLN_HUMAN antibodyEND antibodyEndoglin antibodyENG antibodyFLJ41744 antibodyHHT1 antibodyORW antibodyORW1 antibodyOsler Rendu Weber syndrome 1 antibodyRP11 228B15.2 antibodySN6 antibody
Anti-CD105 antibody [MEM-229] images
ab53318 staining CD105 in infarcted porcine heart by Immunohistochemistry (Frozen sections). Cell nuclei were counterstained blue with DAPI.
ab69772 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab69772 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-CD105 antibody [MEM-229] (ab69772)
ab69772 has not yet been referenced specifically in any publications.