Anti-CD105 antibody [MEM-229] (ab69772)

Overview

• Product nameAnti-CD105 antibody [MEM-229]See all CD105 primary antibodies ...
• Description
  Mouse monoclonal [MEM-229] to CD105
• Tested applicationsICC, IHC-Fr, Flow Cyt, WB more details
• Species reactivity
  Reacts with: Human, Pig
  
• Immunogen

  Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA.

• Positive controlKg1 human acute myelogenous leukemia cell line. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 15mM Sodium Azide
  Constituents: PBS, pH 7.4
• Concentration information loading...
• PurityProtein A purified
• Purification notesab69772 was purified from ascites. Purity is greater than 95% by SDS-PAGE.
• Clonality Monoclonal
• Clone numberMEM-229
• IsotypeIgG2a
• Research Areas
  • Stem Cells
  • Mesenchymal Stem Cells
  • Surface Molecules

  • Stem Cells
  • Endothelial Progenitors
  • Endothelial Markers

  • Cancer
  • Tumor biomarkers
  • Other

  • Cardiovascular
  • Vasculature
  • Endothelium

  • Cardiovascular
  • Cardiovascular Markers
  • Cell Markers
  • Endothelial Cells

  • Immunology
  • Cell Type Markers
  • CD
  • Endothelial Cells

Applications

Target

• FunctionMajor glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
• Tissue specificityEndoglin is restricted to endothelial cells in all tissues except bone marrow.
• Involvement in diseaseDefects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
• Cellular localizationMembrane.

Target information above from: UniProt accession P17813 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2022 Human
  • Omim: 131195 Human
  • SwissProt: P17813 Human
  • Unigene: 76753 Human

• Alternative names
  CD 105 antibodyCD105 antibodyCD105 antigen antibody

  EGLN_HUMAN antibodyEND antibodyEndoglin antibodyENG antibodyFLJ41744 antibodyHHT1 antibodyORW antibodyORW1 antibodyOsler Rendu Weber syndrome 1 antibodyRP11 228B15.2 antibodySN6 antibody

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Anti-CD105 antibody [MEM-229] images

• 

  Immunocytochemistry/ Immunofluorescence - CD105 antibody [MEM-229] (FITC) (ab53318)
  ab53318 staining CD105 in infarcted porcine heart by Immunohistochemistry (Frozen sections). Cell nuclei were counterstained blue with DAPI.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-229] (ab69772)

  ab69772 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab69772 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.