Overview

  • Product name
    Anti-CD105 antibody [SN6]
    See all CD105 primary antibodies
  • Description
    Mouse monoclonal [SN6] to CD105
  • Specificity
    ab11414 recognises the human CD105 cell surface antigen.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IP, WB, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human CD105.

  • General notes
    This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. Use 10ul of the neat dilution to label 106 cells in 100ul.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 75 kDa. CD105 is a glycoprotein homodimer of 95kD subunits.
ICC/IF 1/1000.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function
    Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • Tissue specificity
    Endoglin is restricted to endothelial cells in all tissues except bone marrow.
  • Involvement in disease
    Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI528660 antibody
    • AI662476 antibody
    • CD 105 antibody
    • CD105 antibody
    • CD105 antigen antibody
    • EGLN_HUMAN antibody
    • END antibody
    • Endoglin antibody
    • Eng antibody
    • FLJ41744 antibody
    • HHT1 antibody
    • ORW antibody
    • ORW1 antibody
    • Osler Rendu Weber syndrome 1 antibody
    • RP11 228B15.2 antibody
    • S-endoglin antibody
    • SN6 antibody
    see all

Anti-CD105 antibody [SN6] images

  • ab11414 staining CD105 in human KG1 cells by Flow Cytometry analysis.
  • ab11414 staining CD105 in Human breast tissue by Immunohistochemistry (Frozen sections). The sections were fixed in methanol prior to blocking with 10% serum for 5 minutes at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour. An HRP-conjugated goat anti-mouse polyclonal was used as the secondary antibody.
    Stains exclusively in the stroma - endothelium of the vessels - in human breast tissue. The image shows epithelial breast gland tissue surrounded by stroma.

    See Abreview

  • IHC - Wholemount of rat brain postnatal sections labelling CD105 with ab11414. Sample was incubated with primary antibody (1/200) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated rabbit anti-mouse IgG monoclonal (1/2000) was used as the secondary antibody.

    See Abreview

References for Anti-CD105 antibody [SN6] (ab11414)

This product has been referenced in:
  • Morsing M  et al. Evidence of two distinct functionally specialized fibroblast lineages in breast stroma. Breast Cancer Res 18:108 (2016). Human . Read more (PubMed: 27809866) »
  • Jiao S  et al. The kinase MST4 limits inflammatory responses through direct phosphorylation of the adaptor TRAF6. Nat Immunol 16:246-57 (2015). Read more (PubMed: 25642822) »

See all 11 Publications for this product

Product Wall

Application
IHC - Wholemount
Sample
Rat Tissue (brain postnatal sections)
Specification
brain postnatal sections
Username

Dr. nic savaskan

Verified customer

Submitted Mar 31 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: High pH, Neutral, Low pH
Permeabilization
Yes - 0.25% Triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.2% Triton
Blocking step
Goat serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 02 2012

Thank you very much for your calls today and for letting us know about the trouble with ab44967 and these other CD105 antibodies.

As we discussed, I'm sending a free of charge vial of ab11414 on the order ***, which should arrive tomorrow. Pl...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience th...

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DISCOUNT CODE: XXXXXXXXXXXXXXX
Expiration date: 18-08-2012

DISCOUNT CODE: XXXXXXXXXXXXXXX
Expiration date: 18-08-2012

I am very pleased to hear you would like to accept our offer and test ab11415 and ab11155in [Sheep]. Th...

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (Adipose and bone marrow stem cells)
Specification
Adipose and bone marrow stem cells
Fixative
Paraformaldehyde
Permeabilization
Yes - TRITON x100 0,1%
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Username

Ms. Vega Villar

Verified customer

Submitted Mar 29 2012

Thank you for your reply.

For dilution of the antibodies ready to use in the experiment, as discussed in the previous email I can suggest to dilute antibodiesin PBS orTBS. It is sometimes helpful to include 0.2% Tween 20, however this may ne...

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Thank you for contacting us. In general, the immunoglobulin class (IgG1, IgG2a, IgGM, etc.) and light chain sub-class (kappa or lambda) is listed on the online datasheet. When the light chain is not listed, then the light chain sub-class has not be...

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1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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