Anti-CD105 antibody (ab21224)

Overview

  • Product nameAnti-CD105 antibodySee all CD105 primary antibodies ...
  • Description
    Rabbit polyclonal to CD105
  • Tested applicationsSandwich ELISA, ELISA, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure recombinant human soluble CD105 (aas 26-586) bearing C-terminal His 6 tag.

Properties

Applications

Our Abpromise guarantee covers the use of ab21224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [MEM-226] to CD105 (ab2529). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [MEM-226] to CD105 (ab2529) as Capture.
ELISA Use a concentration of 1 - 15 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 71 kDa.
Flow Cyt Use at an assay dependent concentration.

Target

  • FunctionMajor glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • Tissue specificityEndoglin is restricted to endothelial cells in all tissues except bone marrow.
  • Involvement in diseaseDefects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI528660 antibody
    • AI662476 antibody
    • CD 105 antibody
    • CD105 antibody
    • CD105 antigen antibody
    • EGLN_HUMAN antibody
    • END antibody
    • Endoglin antibody
    • ENG antibody
    • FLJ41744 antibody
    • HHT1 antibody
    • ORW antibody
    • ORW1 antibody
    • Osler Rendu Weber syndrome 1 antibody
    • RP11 228B15.2 antibody
    • S-endoglin antibody
    • SN6 antibody
    see all

Anti-CD105 antibody images

  • Standard Curve for CD105 (Analyte: CD105 protein (ab54338)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [MEM-226] to CD105 (ab2529) at 5ug/ml and Detector Antibody Rabbit polyclonal to CD105 (ab21224) at 0.5ug/ml.
  • Flow Cytometry analysis of CD105 in human endothelial precursor cells using ab21224 undiluted. The secondary used was an undiluted Allophycocyanin conjugated goat anti-rabbit polyclonal.
    Red line= ab21224
    Black line= isotype control

    See Abreview

Protocols

References for Anti-CD105 antibody (ab21224)

ab21224 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (endothelial precursor cell)
Specification endothelial precursor cell
Fixation none
Permeabilization No
Gating Strategy CD133 and CD202B
Username

Dr. A Jackson

Verified customer

Submitted Dec 05 2011

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab21224 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"