Overview

  • Product nameAnti-CD105 antibody, predilutedSee all CD105 primary antibodies ...
  • Description
    Rabbit polyclonal to CD105, prediluted
  • SpecificityWe have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsIHC-P, IHC-Glut, ICC/IF more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 26-176 of Human CD105.

  • Positive control
    • Human tonsil for Immunohistochemistry. IF/ICC: HeLa cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab27422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Ready to use. 30 min at room temperature.
IHC-Glut Use at an assay dependent concentration. PubMed: 22886085
ICC/IF Use at an assay dependent concentration.

Recommended to use neat.

Target

  • FunctionMajor glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • Tissue specificityEndoglin is restricted to endothelial cells in all tissues except bone marrow.
  • Involvement in diseaseDefects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession P17813 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • AI528660 antibody
    • AI662476 antibody
    • CD 105 antibody
    • CD105 antibody
    • CD105 antigen antibody
    • EGLN_HUMAN antibody
    • END antibody
    • Endoglin antibody
    • ENG antibody
    • FLJ41744 antibody
    • HHT1 antibody
    • ORW antibody
    • ORW1 antibody
    • Osler Rendu Weber syndrome 1 antibody
    • RP11 228B15.2 antibody
    • S-endoglin antibody
    • SN6 antibody
    see all

Anti-CD105 antibody, prediluted images

  • ICC/IF image of ab27422 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27422, neat) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Ab27422, prediluted, staining CD105 in formalin fixed, paraffin embedded human tonsil tissue by Immunohistochemistry.

References for Anti-CD105 antibody, prediluted (ab27422)

This product has been referenced in:
  • Virgintino D  et al. Plasma membrane-derived microvesicles released from tip endothelial cells during vascular sprouting. Angiogenesis 15:761-9 (2012). IHC-Glut ; Human . Read more (PubMed: 22886085) »
  • Tront JS  et al. Gadd45a functions as a promoter or suppressor of breast cancer dependent on the oncogenic stress. Cancer Res 70:9671-81 (2010). IHC-P ; Mouse . Read more (PubMed: 21098706) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Tonsil)
Specification Tonsil
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. R Berahovich

Verified customer

Submitted Dec 05 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Unsuitable for use in rat)
Specification Unsuitable for use in rat
Fixative Formaldehyde
Antigen retrieval step Other
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 21 2007

I'm sorry to hear you are experiencing problems with ab27422. Unfortunately I am unable to open the attachment you kindly provided, could you send it again to me? From your comments it seems you are having positive staining plus high background. T...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"