![Flow Cytometry - Anti-CD11+CD18 antibody [24] (ab13219) image](#CD11CD18-Primary-antibodies-ab13219-9.jpg)
![Flow Cytometry - Anti-CD11+CD18 antibody [24] (ab13219) image](#CD11CD18-Primary-antibodies-ab13219-17.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Overview
Product name
Anti-CD11+CD18 antibody [24]
See all CD11+CD18 products (2) ...
Description
Mouse monoclonal [24] to CD11+CD18
Specificity
This antibody specifically recognises the human CD11+CD18 activation epitope.
Tested applications
RIA, IF, Flow Cyt, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Fibronectin-purified human monocytes.
Epitope
Recognises an epitope which is expressed by beta 2 (CD18) integrins in high affinity conformation.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
24
Myeloma
Sp2/0-Ag14
Isotype
IgG1
Research areas
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Immunology >> Cell Type Markers >> CD >> Adhesion
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RIA: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Target
Function
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
Tissue specificity
Leukocytes.
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
Cellular localization
Membrane.
Target information above from: UniProt accessionP20701
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Antigen CD11b antibody
- CD 11b antibody
- CD 18 antibody
see all
Database links
- Entrez Gene: 3683 Human
- Entrez Gene: 3684 Human
- Entrez Gene: 3689 Human
- Entrez Gene: 16408 Mouse
- Entrez Gene: 16409 Mouse
- Entrez Gene: 16414 Mouse
- Omim: 120980 Human
- Omim: 600065 Human
see all
Anti-CD11+CD18 antibody [24] images:
Flow Cytometry - Anti-CD11+CD18 antibody [24] (ab13219)
![Flow Cytometry - Anti-CD11+CD18 antibody [24] (ab13219)](/ps/datasheet/images/13/ab13219/CD11CD18-Primary-antibodies-ab13219-9.jpg)
ab13219 staining CD11 + CD18 in Mouse splenocytes by Flow Cytometry. Cells were prepared in PBS + 1% BSA. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA) for 1 hour at 4°C. A FITC-conjugated Goat anti-mouse polyclonal (1/200) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Flow Cytometry - Anti-CD11+CD18 antibody [24] (ab13219)
![Flow Cytometry - Anti-CD11+CD18 antibody [24] (ab13219)](/ps/datasheet/images/13/ab13219/CD11CD18-Primary-antibodies-ab13219-17.jpg)
ab13219 staining CD11 + CD18 in human PBMC by Flow Cytometry. Cells were prepared in PBS + 1% BSA. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA) for 1 hour at 4°C. An FITC-conjugated Goat anti-mouse polyclonal (1/200) was used as the secondary antibody.
Image courtesy of an anonymous Abreview.
References for Anti-CD11+CD18 antibody [24] (ab13219)
This product has been referenced in:
- Robert Pet al. A novel leukocyte adhesion deficiency III variant: kindlin-3 deficiency results in integrin- and nonintegrin-related defects in different steps of leukocyte adhesion. J Immunol 186:5273-83 (2011). Flow Cyt; Human.Read more (PubMed: 21441448) »
- Asai Aet al. Phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to intracellular adhesion molecule-1. J Lipid Res 50:957-65 (2009). Flow Cyt; Human.Read more (PubMed: 19114730) »
See all 7 publications for this product
Publishing research using ab13219? Please let us know so that we can cite the reference in this datasheet
![Anti-CD11+CD18 antibody [24] for Flow Cytometry in Human (13219)](/ps/datasheet/images/13/ab13219/CD11CD18-Primary-antibodies-ab13219-15.jpg)
![Anti-CD11+CD18 antibody [24] for Flow Cytometry in Mouse (13219)](/ps/datasheet/images/13/ab13219/CD11CD18-Primary-antibodies-ab13219-7.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"