CD11+CD18 antibody [24] (ab13219)

Product Name 

CD11+CD18 antibody [24]

Product type 

Primary antibodies

Description 

Mouse monoclonal [24] to CD11+CD18

Immunogen 

Fibronectin-purified human monocytes.

Reacts with 
(species key)

Hu

Specificity 

This antibody specifically recognises the human CD11+CD18 activation epitope.

Epitope 

Recognises an epitope which is expressed by beta 2 (CD18) integrins in high affinity conformation.

Tested applications 
(see key)

Flow Cyt, ICC/IF, IP, RIA

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
RIA: Use at an assay dependent dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell Membrane

Research areas 

Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Immunology >> Cell Type Markers >> CD >> Adhesion
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha

Relevance 

CD11 (CD11A and CD11B) are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. CD11A is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. CD11B is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles.
CD11A combines with the beta 2 chain (ITGB2 or CD18) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. CD11B combines with the beta 2 chain (ITGB2 or CD18) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3')

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

24

Myeloma 

Sp2/0-Ag14

Isotype 

IgG1

Purity 

IgG fraction

Storage buffer 

Preservative: None
Constituents: PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for CD11+CD18 antibody [24] (ab13219)

  This product has been used in: (two most recent references)

Asai A et al. Phosphatidylcholine hydroperoxide-induced THP-1 cell adhesion to intracellular adhesion molecule-1. J Lipid Res 50:957-65 (2009). Flow Cyt; Human. PubMed: 19114730

Dransfield I et al. Divalent cation regulation of the function of the leukocyte integrin LFA-1. J Cell Biol 116:219-26 (1992). PubMed: 1346139

See all 5 publication references for this product.


If you publish research using ab13219 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding CD11+CD18 antibody [24]

Customer FAQs regarding CD11+CD18 antibody [24]

Protocols for CD11+CD18 antibody [24]

Price and availability of products related to CD11+CD18 antibody [24]

CD11+CD18 antibody [24] - related products: