Anti-CD11a antibody [EP1285Y] (ab52895)

Overview

• Product nameAnti-CD11a antibody [EP1285Y]See all CD11a primary antibodies ...
• Description
  Rabbit monoclonal [EP1285Y] to CD11a
• Tested applicationsIHC-Fr, WB, IP, Flow Cyt, IHC-P, ICC/IF, Blocking more details
• Species reactivity
  Reacts with: Human, Cynomolgus Monkey
  

  Does not react with

  Mouse, Rat
• Immunogen

  Synthetic peptide corresponding to residues near the C terminal of human CD11a.

• Positive controlWB: Jurkat cell lysate. IHC-P: Human spleen tissue.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP1285Y
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Mast Cell Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Lymphoid
  • T Lymphocytic Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Monocytic Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Neutrophil Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Erythrocytic Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Lymphoid
  • NK Cell Lineage

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cell Adhesion
  • Integrins
  • Alpha

  • Immunology
  • Cell Type Markers
  • CD
  • Adhesion

Applications

Target

• FunctionIntegrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
• Tissue specificityLeukocytes.
• Sequence similaritiesBelongs to the integrin alpha chain family.
  Contains 7 FG-GAP repeats.
  Contains 1 VWFA domain.
• DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
• Cellular localizationMembrane.

Target information above from: UniProt accession P20701 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3683 Human
  • Omim: 153370 Human
  • SwissProt: P20701 Human
  • Unigene: 174103 Human

• Alternative names
  Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibodyAntigen CD11A antibodyCD 11a antibody

  CD11 antigen-like family member A antibodyCD11a antibodyCD11a antigen antibodyIntegrin Alpha L antibodyIntegrin alpha-L antibodyIntegrin gene promoter antibodyIntegrin, alpha L (antigen CD11A (p180), lymphocyte function associated antigen 1; alpha polypeptide) antibodyITAL_HUMAN antibodyITGAL antibodyITGAL protein antibodyLeukocyte adhesion glycoprotein LFA 1 alpha chain antibodyLeukocyte adhesion glycoprotein LFA-1 alpha chain antibodyLeukocyte Adhesion Glycoprotein LFA1 Alpha Chain antibodyLeukocyte function associated molecule 1 alpha chain antibodyLeukocyte function-associated molecule 1 alpha chain antibodyLFA 1 alpha antibodyLFA 1 antibodyLFA 1A antibodyLFA-1A antibodyLFA1A antibodyLy15 antibodyLy21 antibodyLymphocyte function associated antigen 1 antibodyLymphocyte Function Associated Antigen Type 1 alpha antibodyp180 antibody

  see all

Anti-CD11a antibody [EP1285Y] images

• 

  Western blot - CD11a antibody [EP1285Y] (ab52895)
  Anti-CD11a antibody [EP1285Y] (ab52895) at 1/20000 dilution + Jurkat cell lysate at 10 µg/ml
  
  Secondary
  Goat anti-rabbit HRP at 1/2000 dilution
  
  Predicted band size : 129 kDa
  Observed band size : 180 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - CD11a antibody [EP1285Y] (ab52895)
  Immunohistochemical staining of paraffin embedded human spleen tissue using ab52895 at 1/100 dilution.
• 

  Flow Cytometry-CD11a antibody [EP1285Y](ab52895)
  Overlay histogram showing Jurkat cells stained with ab52895 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52895, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
  
  
• 

  Immunohistochemistry (Frozen sections) - Anti-CD11a antibody [EP1285Y] (ab52895)Image from Carrillo-de Sauvage MA et al., PLoS One. 2012;7(2):e30762. Epub 2012 Feb 2. Fig S5.; doi:10.1371/journal.pone.0030762; February 2, 2012, PLoS ONE 7(2): e30762.
  Immunohistochemical analysis of cynomolgus monkey brain tissue, staining CD11a with ab52895 at 1/50 dilution.