Overview

  • Product nameAnti-CD11a antibody [EP1285Y]See all CD11a primary antibodies ...
  • Description
    Rabbit monoclonal [EP1285Y] to CD11a
  • Tested applicationsIHC-Fr, WB, IP, Flow Cyt, IHC-P, ICC/IF, Blockingmore details
  • Species reactivity
    Reacts with: Human, Cynomolgus Monkey

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminal of human CD11a.

  • Positive control
    • WB: Jurkat cell lysate. IHC-P: Human spleen tissue.
  • General notesProduced under U.S. Patent No. 5,675,063.

Applications

Our Abpromise guarantee covers the use of ab52895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 22319587
WB 1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 129 kDa).
IP 1/100.
Flow Cyt 1/80 - 1/100.
IHC-P Use at an assay dependent dilution.
ICC/IF 1/100 - 1/250.
Blocking Use at an assay dependent concentration. PubMed: 21540444

Target

  • FunctionIntegrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
  • Tissue specificityLeukocytes.
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
    • Antigen CD11A antibody
    • CD 11a antibody
    • CD11 antigen-like family member A antibody
    • CD11a antibody
    • CD11a antigen antibody
    • Integrin Alpha L antibody
    • Integrin alpha-L antibody
    • Integrin gene promoter antibody
    • Integrin, alpha L (antigen CD11A (p180), lymphocyte function associated antigen 1; alpha polypeptide) antibody
    • ITAL_HUMAN antibody
    • ITGAL antibody
    • ITGAL protein antibody
    • Leukocyte adhesion glycoprotein LFA 1 alpha chain antibody
    • Leukocyte adhesion glycoprotein LFA-1 alpha chain antibody
    • Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain antibody
    • Leukocyte function associated molecule 1 alpha chain antibody
    • Leukocyte function-associated molecule 1 alpha chain antibody
    • LFA 1 alpha antibody
    • LFA 1 antibody
    • LFA 1A antibody
    • LFA-1A antibody
    • LFA1A antibody
    • Ly15 antibody
    • Ly21 antibody
    • Lymphocyte function associated antigen 1 antibody
    • Lymphocyte Function Associated Antigen Type 1 alpha antibody
    • p180 antibody
    see all

Anti-CD11a antibody [EP1285Y] images

  • Anti-CD11a antibody [EP1285Y] (ab52895) at 1/20000 dilution + Jurkat cell lysate at 10 µg/ml

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 129 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical staining of paraffin embedded human spleen tissue using ab52895 at 1/100 dilution.
  • Overlay histogram showing Jurkat cells stained with ab52895 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52895, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Immunohistochemical analysis of cynomolgus monkey brain tissue, staining CD11a with ab52895 at 1/50 dilution.

References for Anti-CD11a antibody [EP1285Y] (ab52895)

This product has been referenced in:
  • Takahara M  et al. Soluble ICAM-1 secretion and its functional role as an autocrine growth factor in nasal NK/T cell lymphoma cells. Exp Hematol 41:711-8 (2013). Read more (PubMed: 23583640) »
  • Carrillo-de Sauvage MA  et al. CCL2-expressing astrocytes mediate the extravasation of T lymphocytes in the brain. Evidence from patients with glioma and experimental models in vivo. PLoS One 7:e30762 (2012). IHC-Fr ; Cynomolgus Monkey . Read more (PubMed: 22319587) »

See all 6 Publications for this product

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