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  • Product nameAnti-CD11a antibody [EP1285Y]
    See all CD11a primary antibodies
  • Description
    Rabbit monoclonal [EP1285Y] to CD11a
  • Tested applicationsIHC-Fr, WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD11a aa 1150 to the C-terminus.
    Database link: P20701

  • Positive control
    • WB: Jurkat and TPH-1 whole cell lyates. IHC-P: Human tonsil, Human squamous cell cervical carcinoma. ICC/IF: Jurkat cell line Flow: Jurkat cell line IP: Jurkat whole cell extract
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-CD11a antibody (Biotin) [EP1285Y] (ab200022)



Our Abpromise guarantee covers the use of ab52895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 22319587
WB 1/5000. Detects a band of approximately 180 kDa (predicted molecular weight: 129 kDa).
IP 1/50.
Flow Cyt 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.


  • FunctionIntegrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
  • Tissue specificityLeukocytes.
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
    • Antigen CD11A antibody
    • CD 11a antibody
    • CD11 antigen-like family member A antibody
    • CD11a antibody
    • CD11a antigen antibody
    • Integrin Alpha L antibody
    • Integrin alpha-L antibody
    • Integrin gene promoter antibody
    • Integrin, alpha L (antigen CD11A (p180), lymphocyte function associated antigen 1; alpha polypeptide) antibody
    • ITAL_HUMAN antibody
    • Itgal antibody
    • ITGAL protein antibody
    • Leukocyte adhesion glycoprotein LFA 1 alpha chain antibody
    • Leukocyte adhesion glycoprotein LFA-1 alpha chain antibody
    • Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain antibody
    • Leukocyte function associated molecule 1 alpha chain antibody
    • Leukocyte function-associated molecule 1 alpha chain antibody
    • LFA 1 alpha antibody
    • LFA 1 antibody
    • LFA 1A antibody
    • LFA-1A antibody
    • LFA1A antibody
    • Ly15 antibody
    • Ly21 antibody
    • Lymphocyte function associated antigen 1 antibody
    • Lymphocyte Function Associated Antigen Type 1 alpha antibody
    • p180 antibody
    see all

Anti-CD11a antibody [EP1285Y] images

  • Immunohistochemical analysis of paraffin-embedded Human squamous cell cervical carcinoma labeling CD11a with ab52895 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab79051) at 1/500 dilution. Membrane/cytoplasmic staining on stromal inflammatory cells of human cervical cancer is observed. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human tonsil labeling CD11a with ab52895 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab79051) at 1/500 dilution. Membrane/cytoplasm staining on lymphocytes of human tonsil is observed. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of cynomolgus monkey brain tissue, staining CD11a with ab52895 at 1/50 dilution.
  • All lanes : Anti-CD11a antibody [EP1285Y] (ab52895) at 1/5000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
    Lane 2 : THP-1 (Human monocytic leukemia cells) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 129 kDa
    Additional bands at : 180 kDa (possible glycosylated form).

    Blocking and Dilution buffer: 5% NFDM/TBST


    The band in THP-1 cells is higher than that in Jurket cells due to the different glycosylation level in different materials.

  • CD11a was immunoprecipitated from 1mg of Jurkat(Human T cell leukemia cells from peripheral blood) whole cell lysates using ab52895 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab52895 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.


  • Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CD11a with ab52895 at 1/50 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line). The unlabeled control is cells without incubation with primary and secondary antibodies (blue line).

References for Anti-CD11a antibody [EP1285Y] (ab52895)

This product has been referenced in:
  • Abramowski P  et al. A truncation variant of the cation channel P2RX5 is upregulated during T cell activation. PLoS One 9:e104692 (2014). WB ; Human . Read more (PubMed: 25181038) »
  • Takahara M  et al. Soluble ICAM-1 secretion and its functional role as an autocrine growth factor in nasal NK/T cell lymphoma cells. Exp Hematol 41:711-8 (2013). Read more (PubMed: 23583640) »

See all 7 Publications for this product

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