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Overview

  • Product nameAnti-CD11a antibody [MEM-83]See all CD11a primary antibodies ...
  • Description
    Mouse monoclonal [MEM-83] to CD11a
  • SpecificityThe antibody MEM-83 reacts with CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
  • Tested applicationsBlocking, Flow Cyt, Functional Studies, IP more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue / cell preparation (Human). Peripheral blood lymphocytes.

  • EpitopeThe antibody recognizes an unique epitope on CD11a antigen (LFA-1 alpha chain) implicated in activation of LFA-1 complex.

Properties

Applications

Our Abpromise guarantee covers the use of ab3981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Blocking BL: Use at an assay dependent concentration. PubMed: 23039186
Flow Cyt Flow Cyt: Use a concentration of 1 µg/ml.
Functional Studies FuncS: Use at an assay dependent concentration. The antibody MEM-83 directly induces the binding of T cells to purified ICAM-1. Using an in vitro-translated CDlla cDNA deletion series, the MEM-83 activation epitope was mapped to the "I" domain of the LFA-1 alpha subunit. The studies have therefore identified a novel LFA-1 activation epitope mapping to the I domain of LFA-1, which could play a role in the regulation of LFA-1 binding to ICAM-1.
IP IP: Use at an assay dependent concentration.

Target

  • FunctionIntegrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
  • Tissue specificityLeukocytes.
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P20701 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibodyAntigen CD11A antibodyCD 11a antibody
        CD11 antigen-like family member A antibodyCD11a antibodyCD11a antigen antibodyIntegrin Alpha L antibodyIntegrin alpha-L antibodyIntegrin gene promoter antibodyIntegrin, alpha L (antigen CD11A (p180), lymphocyte function associated antigen 1; alpha polypeptide) antibodyITAL_HUMAN antibodyITGAL antibodyITGAL protein antibodyLeukocyte adhesion glycoprotein LFA 1 alpha chain antibodyLeukocyte adhesion glycoprotein LFA-1 alpha chain antibodyLeukocyte Adhesion Glycoprotein LFA1 Alpha Chain antibodyLeukocyte function associated molecule 1 alpha chain antibodyLeukocyte function-associated molecule 1 alpha chain antibodyLFA 1 alpha antibodyLFA 1 antibodyLFA 1A antibodyLFA-1A antibodyLFA1A antibodyLy15 antibodyLy21 antibodyLymphocyte function associated antigen 1 antibodyLymphocyte Function Associated Antigen Type 1 alpha antibodyp180 antibody
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    Anti-CD11a antibody [MEM-83] images

    • Blocking - Anti-CD11a antibody [MEM-83] (ab3981)
      Blocking - Anti-CD11a antibody [MEM-83] (ab3981)Image from Ghislin S et al., BMC Cancer. 2012 Oct 5;12:455. doi: 10.1186/1471-2407-12-455. Fig 3.; 5 October 2012, BMC Cancer 2012, 12:455
      Transmigration of A375 Human melanoma cells, blocked using ab3981.

      Melanoma cells were labeled with 10 µM of lipophilic fluorescent dye for 20 minutes at 37°C. Cells were added to wells containing either 1 µg/ml of isotypic control, or 1 µg/ml of anti-CD11a (ab3981) or anti-CD18 (ab8220) antibodies. Melanoma cells were allowed to migrate at 37°C and 5% CO2 for 48 hours. Migration was observed under a fluorescence microscope.

    References for Anti-CD11a antibody [MEM-83] (ab3981)

    This product has been referenced in:
    • Ghislin S  et al. LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro. BMC Cancer 12:455 (2012). Blocking . Read more (PubMed: 23039186) »
    • Choi EY  et al. Regulation of LFA-1-dependent inflammatory cell recruitment by Cbl-b and 14-3-3 proteins. Blood 111:3607-14 (2008). Read more (PubMed: 18239087) »

    See all 5 Publications for this product

    Publishing research using ab3981 ? Please let us know so that we can cite the reference in this datasheet

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    Does this antibody bind the activated LFA-1, or does does it induce activated LFA-1?

    see Landis papers for more details Clone MEM-83 has been reported to uniquely activate LFA-1 to bind ICAM-1. Please see: Landis RC et al. A novel LFA-1 activation epitope maps to the I domain. J Cell Biol 120:1519-27 (1993). PubMed: 7680657 mAb MEM...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"