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ab52478 has been referenced in 5 publications.
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Anti-CD11b antibody [EP1345Y] (ab52478) at 1/20000 dilution + TF1 lysate at 10 µg
Secondary
goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size : 128 kDa
Observed band size : 170 kDa (why is the actual band size different from the predicted?)
Immunohistochemical analysis of paraffin-embedded human spleen using ab52478 at a dilution of 1/100.
Overlay histogram showing RAW 264.7 cells stained with ab52478 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52478, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
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