![Western blot - CD11b antibody [EP1345Y] (ab52478) image](#ab52478_1.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - CD11b antibody [EP1345Y] (ab52478) image](#ab52478_2.jpg)
 image](#CD11b-Primary-antibodies-ab52478-1.jpg)
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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Overview
Product name
Anti-CD11b antibody [EP1345Y]
See all CD11b products (60) ...
Description
Rabbit monoclonal [EP1345Y] to CD11b
Specificity
Testing of Mouse and Rat tissues (brain, spleen, kidney and heart) in WB gave negative results. However, flow cytometry for mouse RAW 264.7 cell line gave positive results. We have not tested any Rat samples in flow cytometry. We welcome any feedback on mouse and rat reactivity.
Tested applications
WB, IP, ICC, Flow Cyt, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding to C terminus of human CD11b
Positive control
IHC-P: Human spleen tissue WB: TF1 lysate
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 0.87% Sodium chloride, 0.09% Tris glycine
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP1345Y
Isotype
IgG
Research areas
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> NK Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Murine Lineage Negative
Stem Cells >> Mesenchymal Stem Cells >> Negative Markers
Neuroscience >> Cell Type Marker >> Glia marker >> Microglia marker
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/20,000 - 1/50,000.Predicted molecular weight: 128 kDa.
IP
IP: 1/80IP: 1/80
ICC
ICC: 1/100 - 1/250.ICC: 1/100 - 1/250.
Flow Cyt: 1/20 - 1/50.
IHC-P: 1/100 - 1/250.(1/100 - 1/250. An antigen retrieval step is recommended.)
Target
Function
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
Tissue specificity
Predominantly expressed in monocytes and granulocytes.
Involvement in disease
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
Cellular localization
Membrane.
Target information above from: UniProt accessionP11215
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
- CD11 antigen-like family member B antibody
see all
Database links
- Entrez Gene: 3684 Human
- Entrez Gene: 16409 Mouse
- Omim: 120980 Human
- SwissProt: P11215 Human
- SwissProt: P05555 Mouse
- Unigene: 172631 Human
- Unigene: 262106 Mouse
Anti-CD11b antibody [EP1345Y] images:
Western blot - CD11b antibody [EP1345Y] (ab52478)
![Western blot - CD11b antibody [EP1345Y] (ab52478)](/ps/datasheet/Images/52/ab52478/ab52478_1.jpg)
Anti-CD11b antibody [EP1345Y] (ab52478) at 1/20000 dilution + TF1 lysate at 10 µg
Secondary
goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size : 128 kDa
Observed band size : 170 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - CD11b antibody [EP1345Y] (ab52478)
![Immunohistochemistry (Paraffin-embedded sections) - CD11b antibody [EP1345Y] (ab52478)](/ps/datasheet/Images/52/ab52478/ab52478_2.jpg)
Immunohistochemical analysis of paraffin-embedded human spleen using ab52478 at a dilution of 1/100.
Flow Cytometry-CD11b antibody [EP1345Y](ab52478)
](/ps/datasheet/images/52/ab52478/CD11b-Primary-antibodies-ab52478-1.jpg)
Overlay histogram showing RAW 264.7 cells stained with ab52478 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52478, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in RAW 264.7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
References for Anti-CD11b antibody [EP1345Y] (ab52478)
This product has been referenced in:
- Xue ZHet al. Integrin alphaMbeta2 clustering triggers phosphorylation and activation of protein kinase Cdelta that regulates transcription factor Foxp1 expression in monocytes. J Immunol 184:3697-709 (2010).Read more (PubMed: 20190138) »
- Blatner NRet al. In colorectal cancer mast cells contribute to systemic regulatory T-cell dysfunction. Proc Natl Acad Sci U S A 107:6430-5 (2010). IHC-P; Human.Read more (PubMed: 20308560) »
See all 5 publications for this product
Publishing research using ab52478? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"