Western blot abreview for Anti-CD11b antibody [EPR1344]

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
20 µg
Specification
Hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST (0.1% Tween 20)
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
Clarity™ Western ECL Substrate
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 170 kDa Non-specific: 36, 38 kDa
Positive control
Mouse spleen whole tissue lysate

Additional data

Additional Notes
Lanes 1 and 2: Mouse hippocampus (whole tissue lysate), 2 different samples. Lane 3 (Positive control): Mouse spleen (whole tissue lysate)
Username

Dr. Sergi Bayod

Verified customer

Submitted Jan 23 2018

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