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Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Mouse Tissue sections (Hippocampus)
Yes - 0.2% Triton in PBS
1% BSA + 5% Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.2% Triton in PBS with 1% BSA
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L)
Host species: Goat
Conjugation: Alexa Fluor® 488
CD11b ab133357 (green) and Hoechst (blue).
Counterstained with Hoechst, 2 ug/ml for 5 min at room temperature.
Mounted in Fluoromount-G.
Dr. Sergi Bayod
Submitted Feb 05 2018