Immunohistochemistry (Frozen sections) abreview for Anti-CD11b antibody [EPR1344]

Average
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Hippocampus)
Permeabilization
Yes - 0.2% Triton in PBS
Specification
Hippocampus
Blocking step
1% BSA + 5% Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Acetone

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.2% Triton in PBS with 1% BSA
Dilution
1/200

Secondary antibody

Dilution
1/250
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
CD11b ab133357 (green) and Hoechst (blue). Counterstained with Hoechst, 2 ug/ml for 5 min at room temperature. Mounted in Fluoromount-G.
Username

Dr. Sergi Bayod

Verified customer

Submitted Feb 05 2018

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