Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11b antibody [EPR1344]

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Skin (epidermis to SQ fat or muscle))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Invitrogen eBioscience IHC antigen retrieval solution, low pH, 10x
Permeabilization
Yes - H2O2
Specification
Skin (epidermis to SQ fat or muscle)
Blocking step
ab64226 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
10% neutral buffered formalin

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: overnight

Secondary antibody

Name
Non-Abcam antibody was used: Abcam antibody was used but it would not let me su
Host species: Goat
Clonality: Polyclonal
Conjugation: Streptavidin

Additional data

Additional Notes
We tried the following dilutions: 1:500, 1:1000, 1:2000, 1:4000. Even when concentrated, there was no apparent staining.
Abcam response
Our Scientific Support team has investigated this negative Abreview, and the issue has been resolved in accordance with our Abpromise.
Username

Abcam user community

Verified customer

Submitted Feb 28 2018

Sign up