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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD11b antibody [M1/70.15] (ab6332)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD11b antibody [M1/70.15] (ab6332)](/ps/datasheet/images/ab6332_1.jpg)
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Immunohistochemistry (Frozen sections) - CD11b antibody [M1/70.15] (ab6332)
![Immunohistochemistry (Frozen sections) - CD11b antibody [M1/70.15] (ab6332)](/ps/datasheet/images/ab6332_2.jpg)
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Product Name
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CD11b antibody [M1/70.15]
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See all CD11b antibodies (55)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [M1/70.15] to CD11b
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Reacts with
(species key)
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Hu, Ms, Rb
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Specificity
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Detects the CD11b antigen (MW170kDa and 95kDa) also known as Mac-1 antigen, found on mouse macrophages, their precursors and granulocytes.
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-FoFr, IHC-Fr, IHC-FrFl, IHC-P, P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/25 - 1/200 (10µl per million cells).
IHC-P: 1/25 - 1/200. Use on PERIODATE LYSINE paraformaldehyde fixed paraffin embedded tissues (the antibody DOES NOT WORK on paraformaldehyde-only fixed), perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Use at an assay dependent dilution.
IHC-FoFr: 1/100.
ICC: Use at an assay dependent dilution.
P: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Type I membrane protein.
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Research areas
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Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> NK Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Murine Lineage Negative
Stem Cells >> Mesenchymal Stem Cells >> Negative Markers
Neuroscience >> Cell Type Marker >> Glia marker >> Microglia marker
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
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Relevance
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CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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M1/70.15
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Isotype
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IgG2b
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol, PBS Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD11b antibody [M1/70.15] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD11b antibody [M1/70.15] images, references, products related to ab6332 and other tools.
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CD11b antibody [M1/70.15] images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD11b antibody [M1/70.15] (ab6332)
This image was kindly supplied as part of the review submitted by JQ Zhang.
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Immunohistochemistry (Frozen sections) - CD11b antibody [M1/70.15] (ab6332)
ab6332 at 1/500 staining mouse lumbar spinal cord tissue sections (30um) by IHC-Fr. The tissue was formaldehyde fixed and blocked with 3% serum, prior to incubation with the antibody for 18 hours. A biotinylated goat anti-rat polyclonal antibody was used as the secondary. This image is courtesy of an Abreview submitted by Ms Nancy Nutile-McMenemy
See Abreview
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References for CD11b antibody [M1/70.15] (ab6332)
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This product has been used in: (two most recent references)
Palazuelos J et al. The CB2 Cannabinoid Receptor Controls Myeloid Progenitor Trafficking: INVOLVEMENT IN THE PATHOGENESIS OF AN ANIMAL MODEL OF MULTIPLE SCLEROSIS. J Biol Chem 283:13320-9 (2008). IHC-P; Human. PubMed: 18334483
Wieghaus KA et al. Expansion of microvascular networks in vivo by phthalimide neovascular factor 1 (PNF1). Biomaterials 29:4698-708 (2008). IHC-FoFr; Mouse. PubMed: 18804278
See all 14 publication references for this product.
If you publish research using ab6332 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to CD11b
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CD11b antibody [M1/70.15] - more information
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Customer reviews (feedback) regarding CD11b antibody [M1/70.15] |
Customer FAQs regarding CD11b antibody [M1/70.15] |
Protocols for CD11b antibody [M1/70.15] |
Price and availability of products related to CD11b antibody [M1/70.15] |
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CD11b antibody [M1/70.15] - related products:
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CD11b antibody [M1/70.15] - other tools:
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Contact Abcam with a Technical Enquiry about CD11b antibody [M1/70.15] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD11b antibody [M1/70.15] (ab6332)](/index.html?datasheet=6332#ab6332_1.jpg)
![Immunohistochemistry (Frozen sections) - CD11b antibody [M1/70.15] (ab6332)](/index.html?datasheet=6332#ab6332_2.jpg)
