Recombinant Anti-CD11b antibody [SP330] - C-terminal (ab224805)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP330] to CD11b - C-terminal
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD11b antibody [SP330] - C-terminal
See all CD11b primary antibodies -
Description
Rabbit monoclonal [SP330] to CD11b - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: TF-1 cell lysate. IHC-P: Human tonsil, colon, spleen, bone marrow, lung, cerebellum, thymus, lymph node, HK lymphoma, pancreatic adenocarcinoma and endometrial adenocarcinoma tissues. Flow Cyt: TF-1 cells.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP330 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/100.
Primary incubation for 30 minutes at at 4°C. |
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WB |
1/400. Predicted molecular weight: 127 kDa.
Primary incubation for 1 hour at room temperature. |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Primary incubation for 10 minutes at room temperature. |
Notes |
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Flow Cyt
1/100. Primary incubation for 30 minutes at at 4°C. |
WB
1/400. Predicted molecular weight: 127 kDa. Primary incubation for 1 hour at room temperature. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Primary incubation for 10 minutes at room temperature. |
Target
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Function
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. -
Tissue specificity
Predominantly expressed in monocytes and granulocytes. -
Involvement in disease
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3684 Human
- Omim: 120980 Human
- SwissProt: P11215 Human
- Unigene: 172631 Human
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Alternative names
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
see all
Images
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Flow cytometric analysis of TF-1 (human bone marrow erythroleukemia cell line) cell line labeling CD11b with ab224805 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue) .
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Anti-CD11b antibody [SP330] - C-terminal (ab224805) at 1/100 dilution + TF-1 (human bone marrow erythroleukemia cell line) cell lysate
Predicted band size: 127 kDa -
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human spleen tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human bone marrow tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lung tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human cerebellum tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human thymus tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lymph node tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab224805 has been referenced in 1 publication.
- Masugi Y et al. Characterization of spatial distribution of tumor-infiltrating CD8+ T cells refines their prognostic utility for pancreatic cancer survival. Mod Pathol 32:1495-1507 (2019). PubMed: 31186528