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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab75476? Please let us know so that we can cite the reference in this datasheet
ab75476 has been referenced in 3 publications.
- Lawson CD et al. P-rex1 and vav1 cooperate in the regulation of formyl-methionyl-leucyl-phenylalanine-dependent neutrophil responses. J Immunol 186:1467-76 (2011). WB. PubMed: 21178006
- Overbeek SA et al. Cigarette smoke induces ß2-integrin-dependent neutrophil migration across human endothelium. Respir Res 12:75 (2011). IHC-P; Mouse. PubMed: 21651795
- Ramos KM et al. Spinal upregulation of glutamate transporter GLT-1 by ceftriaxone: therapeutic efficacy in a range of experimental nervous system disorders. Neuroscience 169:1888-900 (2010). WB; Rat. PubMed: 20547213
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - CD11b antibody (ab75476)
Anti-CD11b antibody (ab75476) at 2 µg/ml + RAW 264.7 whole cell lysate
Observed band size : ~160 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD11b antibody (ab75476)
ab75476 staining CD11b in mouse spleen tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were blocked, then incubated with the primary antibody at a 1/400 dilution for 20 minutes. An undiluted HRP-conjugated goat anti-rabbit monoclonal was used as secondary antibody.
This image is a courtesy of Anonymous Abreview
Immunocytochemistry/ Immunofluorescence - CD11b antibody (ab75476)
ab75476 staining CD11b in murine bronchoalveolar lavage cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in methanol. Samples were then incubated with ab75476 at a 1/4000 dilution for 16 hours at 4°C. The secondary used was an undiluted goat anti-rabbit polyclonal conjugated to biotin. Streptavidin Alexa 488 (green)(1/500) was used to amplify the signal.
Image courtesy of Dr Francois Daubeuf by Abreview.
CD11b antibody for WB in Mouse (75476)
CD11b antibody for IHC-P in Mouse (75476)
CD11b antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (75476)
CD11b antibody for Immunocytochemistry/ Immunofluorescence in Mouse (75476)
Anti-CD11b antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (75476)
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