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Flow Cytometry - CD11b antibody (ab8878)
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Immunohistochemistry (Frozen sections) - CD11b antibody (ab8878)
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Product Name
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CD11b antibody
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See all CD11b antibodies (59)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal to CD11b
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Immunogen
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The hybridoma was formed by the fusion of mouse myeloma NS1 cellswith spleen cells from rats immunized with B10 mouse spleen cells enrichedfor T lymphocytes.
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Reacts with
(species key)
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Hu, Ms, Rb
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Specificity
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This antibody recognises Mac-1 (CD11b)
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Tested applications
(see key)
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Flow Cyt, IHC-Fr, IP
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-Fr: Use at an assay dependent dilution (PMID 18469117).
Immunoprecipitation: The monoclonal antibody and polyclonal antisera weremixed with labeled cell lysates or purified antigens for 2 hours at 4°C followed bythe addition of 40 ml purified RG7/7.6 mouse anti-rat kappa chain coupled tosepharose 2 mg/ml.
After shaking for 1 1/2 hours at 4°C, samples were washedand subjected to SDS-PAGE and autoradiography with enhancing screens (1).
FACS analysis: Each labeling step was carried out for 30 min at 0-4°C in mediumcontaining 0.01 M NaN3.
Cells (5 x 107/ml) were incubated with 50 µl M1 monoclonal antibody or irrelevant monoclonal antibody, R4/18.2, as control in the first step, washed, suspended in 50 µl of FITC-F (ab')2 anti-rat IgGin the second step, and washed through a layer of fetal calf serum.
Can also be used in cytotoxicity and binding assays.
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Cellular localization
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Type I membrane protein.
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Research areas
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Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> NK Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Murine Lineage Negative
Stem Cells >> Mesenchymal Stem Cells >> Negative Markers
Neuroscience >> Cell Type Marker >> Glia marker >> Microglia marker
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
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Relevance
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CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rat
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Clonality
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Monoclonal
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Myeloma
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NS1
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Isotype
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IgG2b
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Purity
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Ascites
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Storage buffer
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PBS, pH 7.4, no preservative, sterile filtered
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Form
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Liquid
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Concentration
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1.800 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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CD11b is also known as MAC-1alpha, CR3 and integrin alpha M.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD11b antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD11b antibody images, references, products related to ab8878 and other tools.
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CD11b antibody images:
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 Flow Cytometry - CD11b antibody (ab8878)
Ab8878 on human macrophages isolated from whole blood. (red - result with 95% expression, yellow - negative control)
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Immunohistochemistry (Frozen sections) - CD11b antibody (ab8878)
ab8878 staining CD11b in mouse brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocked with 5% BSA for 1 hour at 250C. The sample was incubated with primary antibody (1/100) for 12 hours at 40C in PBS. A Cy3®-conjugated donkey polyclonal to rat IgG (1/500) was used as secondary antibody. Red staining in the image represents Cy3-CD11b, green - FITC-BrdU and blue is DAPI staining. This image is a courtesy of Anonimous Abreview
See Abreview
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References for CD11b antibody (ab8878)
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This product has been used in: (two most recent references)
Tamagawa E et al. Particulate matter exposure induces persistent lung inflammation and endothelial dysfunction. Am J Physiol Lung Cell Mol Physiol 295:L79-85 (2008). IHC-Fr; Rabbit. PubMed: 18469117
Sanchez-Madrid F et al. Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med 158:586-602 (1983). PubMed: 6193226
See all 3 publication references for this product.
If you publish research using ab8878 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to CD11b
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CD11b antibody - more information
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