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Overview

  • Product nameCD130 Human ELISA KitSee all CD130 (gp130) kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 8 1751pg/ml 17.61 1%
    B 8 264.3pg/ml 8.67 3.27%
    Inter-assay
    Sample n Mean SD CV%
    A 16 1782pg/ml 26.64 1.49%
    B 16 247pg/ml 11.99 4.85%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 15 pg/ml
  • Range
    56.25 pg/ml - 1800 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Serum 97 % - %

  • Assay time
    1h 40m
  • Species reactivity
    Reacts with: Human
  • Product overview

    INTENDED USE

    The Human CD130 ELISA is to be used for the in-vitro quantitative determination of soluble CD130 protein (gp130) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human CD130. This kit has been configured for research use only and is not to be used in diagnostic procedures.

    PRINCIPLE OF THE METHOD

    The CD130 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD130 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD130 concentrations, control specimens and unknowns are pipetted into these wells.

    During the first incubation, the CD130 antigen and a biotinylated monoclonal antibody specific for CD130 are simultaneously incubated.

    After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD130 present in the samples.

     

    Recognizes both natural and recombinant human CD130

    Range: 56.25 pg/ml - 1800 pg/ml

    Sensitivity <15 pg/ml

  • Notes

    Safety Information:

    • For research use only.

    • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.

    • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.

    • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.

    • Do not pipette by mouth.

    • The stop solution in this kit contains 9.8% sulphuric acid.

  • Tested applicationsELISA more details

Properties

  • RelevanceCD130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.
  • Cellular localizationCell membrane; single-pass type I membrane protein and secreted
    • Alternative names
        CD130 antigenCDw130 gp130
        IL6 R betaInterleukin 6 receptor subunit beta Interleukin 6 signal transducerMembrane glycoprotein 130Oncostatin M receptor alpha subunit
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab46135 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      CD130 Human ELISA Kit images

      • ELISA - Human CD130 ELISA kit (ab46135)
        ELISA - Human CD130 ELISA kit (ab46135)
        Typical CD130 standard curve ranging from 56.25 to 1800pg/mL

      References for CD130 Human ELISA Kit (ab46135)

      ab46135 has not yet been referenced specifically in any publications.

      Publishing research using ab46135 ? Please let us know so that we can cite the reference in this datasheet

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"