Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-CD130 (gp130) antibody [B-S12] - Azide free
See all CD130 (gp130) products (18) ...
Description
Mouse monoclonal [B-S12] to CD130 (gp130) - Azide free
Specificity
This antibody reacts with CD130 (gp130).
Tested applications
WB, IP, Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length protein (Human): Natural soluble gp130 receptor.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purification notes
This antibody was purified by ion exchange chromatography. It is supplied sterile filtered through 0.22 µm, and has been treated to remove endotoxins.
Primary antibody notes
This antibody activates cells expressing CD130. It also induces the phosphorylation of CD130, Jak 1-2, and STAT 3.
Clonality
Monoclonal
Clone number
B-S12
Myeloma
x63-Ag8.653
Isotype
IgG1
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Heart >> Apoptosis
Cardiovascular >> Atherosclerosis >> Apoptosis
Cancer >> Signal transduction >> Other
Signal Transduction >> Adapters >> Transmembrane
Immunology >> Cell Type Markers >> CD >> Cytokines
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab34322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 103 kDa.
IP: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
Target
Function
Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.
Tissue specificity
Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
Sequence similarities
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
Post-translational
modifications
Phosphorylation of Ser-782 down-regulates cell surface expression.
Heavily N-glycosylated.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionP40189
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD130 antibody
- CD130 antigen antibody
- CDw130 antibody
see all
Database links
- Entrez Gene: 3572 Human
- Omim: 600694 Human
- SwissProt: P40189 Human
- SwissProt: Q5FC07 Human
- Unigene: 532082 Human
- Unigene: 706627 Human
References for Anti-CD130 (gp130) antibody [B-S12] - Azide free (ab34322)
This product has been referenced in:
- Wijdenes Jet al. Interleukin-6 signal transducer gp130 has specific binding sites for different cytokines as determined by antagonistic and agonistic anti-gp130 monoclonal antibodies. Eur J Immunol 25:3474-81 (1995).Read more (PubMed: 8566040) »
See 1 publication for this product
Publishing research using ab34322? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"