![Flow Cytometry / FACS - CD130 (gp130) antibody [B-S12] (ab27359) image](#ab27359.gif)
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Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-CD130 (gp130) antibody [B-S12]
See all CD130 (gp130) products (18) ...
Description
Mouse monoclonal [B-S12] to CD130 (gp130)
Specificity
Recognises the Gp130, common subunit for IL-6, IL-11, OSM, LIF, CNTF, CT-1 receptors and soluble antigen. Activates cells carrying gp130.
Tested applications
WB, IP, Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
Natural soluble gp130
Positive control
Eahy 926 cell line.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ion Exchange Chromatography
Purification notes
ab27359 is purified by ion exchange chromatography and sterile filtered through 0.22µm and treated to remove endotoxins.
Primary antibody notes
Induces gp130 activation, Jak 1, Jak 2, Stat 1 and Stat 3 phosphorylation.
Clonality
Monoclonal
Clone number
B-S12
Isotype
IgG1
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Heart >> Apoptosis
Cardiovascular >> Atherosclerosis >> Apoptosis
Cancer >> Signal transduction >> Other
Signal Transduction >> Adapters >> Transmembrane
Immunology >> Cell Type Markers >> CD >> Cytokines
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
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Flow Cytometry / FACS - CD130 (gp130) antibody [B-S12] (ab27359)
![Flow Cytometry / FACS - CD130 (gp130) antibody [B-S12] (ab27359) image](/ps/datasheet/Images/27/ab27359/ab27359.gif)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab27359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 130 kDa.
IP: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
Target
Function
Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.
Tissue specificity
Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
Sequence similarities
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
Post-translational
modifications
Phosphorylation of Ser-782 down-regulates cell surface expression.
Heavily N-glycosylated.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionP40189
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD130 antibody
- CD130 antigen antibody
- CDw130 antibody
see all
Database links
- Entrez Gene: 3572 Human
- Omim: 600694 Human
- SwissProt: P40189 Human
- SwissProt: Q5FC07 Human
- Unigene: 532082 Human
- Unigene: 706627 Human
Anti-CD130 (gp130) antibody [B-S12] images:
Flow Cytometry / FACS - CD130 (gp130) antibody [B-S12] (ab27359)
A typical staining pattern with ab27359 using Eahy 926 cell line.
References for Anti-CD130 (gp130) antibody [B-S12] (ab27359)
This product has been referenced in:
- Fourcin Met al. gp130 transducing receptor cross-linking is sufficient to induce interleukin-6 type responses. J Biol Chem 271:11756-60 (1996).Read more (PubMed: 8662709) »
See 1 publication for this product
Publishing research using ab27359? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"