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Anti-CD130 (gp130) antibody (ab14491)

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Overview

  • Product nameAnti-CD130 (gp130) antibodySee all CD130 (gp130) primary antibodies ...
  • Description
    Rabbit polyclonal to CD130 (gp130)
  • Tested applicationsWB, IP more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fusion protein: FKKEKIN TEGHSSGIGG SSCMSSSRPS ISSSDENESS QNTSSTVQYS TVVHSGYRHQ VPSVQVFSRS ESTQPLLDSE ERPEDLQLVD HVDGGDGILP RQQYFKQNCS QHESSPDISH FERSKQVSSV NEEDFVRLKQ QISDHISQSC GSGQMKMFQE VSAADAFGPG TEGQVERFET VGMEAATDEG MPKSYLPQTV RQGGYMPQ , corresponding to intracellular amino acids 713-918 of Human CD130 (gp130).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control3T3 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab14491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 130 kDa.
IP IP: Use at an assay dependent dilution.

Target

  • FunctionSignal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.
  • Tissue specificityFound in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 5 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Post-translational
    modifications    Phosphorylation of Ser-782 down-regulates cell surface expression.
    Heavily N-glycosylated.
  • Cellular localizationSecreted and Cell membrane.

    • Target information above from: UniProt accession P40189 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        CD130 antibodyCD130 antigen antibodyCDw130 antibody
        gp130 antibodyGP130 RAPS antibodygp130 transducer chain antibodyGP130-RAPS antibodyIL 6R beta antibodyIL-6 receptor subunit beta antibodyIL-6R subunit beta antibodyIL-6R-beta antibodyIL-6RB antibodyIL6 ST antibodyIL6R-beta antibodyIL6RB_HUMAN antibodyIL6ST antibodyInterleukin 6 receptor subunit beta antibodyInterleukin 6 signal transducer antibodyInterleukin receptor beta chain antibodyInterleukin-6 receptor subunit beta antibodyInterleukin-6 signal transducer antibodyMembrane glycoprotein 130 antibodyMembrane glycoprotein gp130 antibodyOncostatin M receptor alpha subunit antibodyOncostatin M receptor antibodyOncostatin-M receptor subunit alpha antibody
      see all

    References for Anti-CD130 (gp130) antibody (ab14491)

    ab14491 has not yet been referenced specifically in any publications.

    Publishing research using ab14491 ? Please let us know so that we can cite the reference in this datasheet

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    I do in fact have another question with regard to an anti-CD130 (gp130) antibody (catalog number: ab14491) that I purchased approximately 2-3 weeks ago. I am actually wondering how to prepare the 3T3 cell lysate that is listed as the positive control as p...

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    Assuming you are preparing a lysate for western blotting, I recommend consulting our western blotting guide at the following link, in particular the Sample Preparation section. A standard RIPA buffer with protease inhibitors will be suitable for preparing...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"