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Overview

  • Product nameCD14 Human ELISA KitSee all CD14 kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 6 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    74 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 73.77 67% - 81%
    Serum 76.23 70% - 85%
    Plasma 73.87 67% - 80%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam's Human CD14 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD14 in serum, plasma and cell culture supernatants.

     

     This assay employs an antibody specific for Human CD14 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD14 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CD14 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD14 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    Biotinylated anti-human CD14 2 vials
    CD14 Microplate (12 strips x 8 wells) 1 unit
    CD14 Standard Human(recombinant) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research Areas
  • RelevanceCD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock.
    • Alternative names
        CD 14CD14 antigenCD14 molecule
        Monocyte differentiation antigen CD14Myeloid cell-specific leucine-rich glycoprotein
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab99989 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      CD14 Human ELISA Kit images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab99989.

      Protocols

      References for CD14 Human ELISA Kit (ab99989)

      ab99989 has not yet been referenced specifically in any publications.

      Publishing research using ab99989 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab99989 Submit a Question for ab99989

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      a buffer blank is included on each plate. It is the wells below the lowest point on the standard curve.

      Thanks for your help,

      Thank you very much for the clarification. Please see below for the details of your refund.

      Your credit note ID is ***

      I am sorry that thiskit did not perform as stated on the datasheet. If payment has already been made on the original ...

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      Thanks for your help
      I have attached the test I ran on the new kits. I only ran the standard curve because we wanted to be sure of the kits before running test samples on them.

      The first set of files I sent had the background wavelength of...

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      Thank you again for sending all of this data and for your patience. I am working with my colleagues to set up this refund for you.

      I apologize for my confusion regarding the background. I hadn't realized this was a reference wavelength reading. ...

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      Thanks for your help. Here are the data without background.

      I tested the new batch of CD14 ELISAs and go the same, very low to negative results. Because of this, we have decided that we are no longer interested in pursuing CD14 and would like a...

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      Thank you for your emails, and I am sorry to hear that the replacement kits are also not providing the expected results.

      Could you tell me how many of the replacement kits were tried, and were the samples and standards both giving low results ag...

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      thanks for getting back to me

      The OD values are after background subtraction.

      These kits we stored at 4 oC since we received them. About 3 weeks.

      The SA-HRP is prepared per the protocol. It is allowed to come to room temp,...

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      Thank you again for sending all of the data.

      I have heard back from the lab, and they are wondering if you could send the OD values before background subtraction (or just indicate the value of background)? The lab scientists would just like to c...

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      I think the attachments got lost at some point. Could you please resend them?

      Thanks,

      Thank you for letting me know. I apologize if I forgot to attach the data! Please see the file attached.

      I have also received the additional information that you sent in another email, and I will forward that on to the lab. Please let me know if...

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      Could I see a standard curve that you have produced in your lab? I have used the kit in the past and seen low OD values for the standards.

      Thank you for your calls earlier this week and for your patience while I have been in touch with the lab regarding your enquiry about ab99989.

      I have obtained the standard OD values determined during the QC testing for the two lots that you have...

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      Could I see a standard curve that you have produced in your lab? I have used the kit in the past and seen low OD values for the standards.

      Thank you for your calls last week and for your patience while I have been in touch with the lab regarding your enquiries. Thank you also for sending your data from the most recent assays with the kits.
      I have requested to see the standard curve data...

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      Standard curve was completely flat using two kits from this order.

      Thank you for your call today and for letting us know about the problem with these kits.

      As we discussed, I'm sending free of charge replacement kits on the order ***, which I expect to arrive by the beginning of next week.

      If you have...

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      Phone call requesting information of what dilution to use human plasma samples with the CD14 Human ELISA Kit as well as how likely it is to react with mouse samples.

      Thank you for contacting us yesterday in regards to the CD14 Human ELISA Kit (ab99989).

      I cannow confirmthe detailsyourequested.We wouldrecommendedusing between a 2,000-20,000 fold dilution for human plasma. Therefore startingwith a 1/5000 dilut...

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      Hei,
      thanks for the reply. what is the difference between these kits? Anyway, i would like to order the products with two plates CD14 Human ELISA Kit (ab45523). could you kindly deliver as soon as possible. I have sent the details concerning the bill...

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      I am sorry for the delay in getting back to you.

      As discussed in my previous email, the human CD14 ELISA kits ab99989 and ab45523 are both sandwich ELISA kits which bind CD14 to the surface of the plate and use another antibody to detect the bou...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"