Anti-CD14 antibody [5A3B11B5] (ab36595)

Overview

  • Product nameAnti-CD14 antibody [5A3B11B5]
    See all CD14 primary antibodies
  • Description
    Mouse monoclonal [5A3B11B5] to CD14
  • Tested applicationsIHC-P, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Ni-NTA purified recombinant human CD14 expressed in E. Coli strain BL21 (DE3).

  • Positive control
    • Human lymph node normal tissue.
  • General notesAb81216-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab36595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.01µg for 106 cells.

Target

  • FunctionCooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • Tissue specificityExpressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • Sequence similaritiesContains 11 LRR (leucine-rich) repeats.
  • Post-translational
    modifications
    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 14 antibody
    • CD_antigen=CD14 antibody
    • CD14 antibody
    • CD14 antibody
    • CD14 antigen antibody
    • CD14 antigen antibody
    • CD14 molecule antibody
    • CD14_HUMAN antibody
    • Lipopolysaccharide receptor antibody
    • Lipopolysaccharide receptor antibody
    • LPS-R antibody
    • LPSR antibody
    • LPSR antibody
    • Mo2 antibody
    • Monocyte Differentiation Antigen 14 antibody
    • Monocyte Differentiation Antigen 14 antibody
    • Monocyte differentiation antigen CD14 antibody
    • Monocyte differentiation antigen CD14 urinary form antibody
    • Monocyte differentiation antigen CD14, membrane-bound form antibody
    • Myeloid cell specific leucine rich glycoprotein antibody
    • Myeloid cell-specific leucine-rich glycoprotein antibody
    see all

Anti-CD14 antibody [5A3B11B5] images

  • Human peripheral blood cells stained with ab36595 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab36595, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Ab36595 at a 1/1300 dilution (Figure 1), and normal mouse serum at a 1/20 dilution (Figure 2), staining paraffin embedded human normal lymph node by immunohistochemistry. HRP-anti-mouse was used as the secondary antibody before colour development with DAB.

References for Anti-CD14 antibody [5A3B11B5] (ab36595)

ab36595 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your further questions.

I can confirm the following prices:
CD14 antibody [5A3B11B5] (ab36595) 100 ul £227.00
CD14 antibody [7] (ab49755) 200 ug £227.00
Delivery: £16.00 to United Kingdom

This informat...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"