Anti-CD14 antibody [5A3B11B5] (ab36595)

Overview

  • Product nameAnti-CD14 antibody [5A3B11B5]
    See all CD14 primary antibodies
  • Description
    Mouse monoclonal [5A3B11B5] to CD14
  • Tested applicationsIHC-P, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Ni-NTA purified recombinant human CD14 expressed in E. Coli strain BL21 (DE3).

  • Positive control
    • Human lymph node normal tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab36595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.01µg for 106 cells.

Target

  • FunctionCooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • Tissue specificityExpressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • Sequence similaritiesContains 11 LRR (leucine-rich) repeats.
  • Post-translational
    modifications
    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 14 antibody
    • CD_antigen=CD14 antibody
    • CD14 antibody
    • CD14 antibody
    • CD14 antigen antibody
    • CD14 antigen antibody
    • CD14 molecule antibody
    • CD14_HUMAN antibody
    • Lipopolysaccharide receptor antibody
    • Lipopolysaccharide receptor antibody
    • LPS-R antibody
    • LPSR antibody
    • LPSR antibody
    • Mo2 antibody
    • Monocyte Differentiation Antigen 14 antibody
    • Monocyte Differentiation Antigen 14 antibody
    • Monocyte differentiation antigen CD14 antibody
    • Monocyte differentiation antigen CD14 urinary form antibody
    • Monocyte differentiation antigen CD14, membrane-bound form antibody
    • Myeloid cell specific leucine rich glycoprotein antibody
    • Myeloid cell-specific leucine-rich glycoprotein antibody
    see all

Anti-CD14 antibody [5A3B11B5] images

  • Human peripheral blood cells stained with ab36595 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab36595, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Ab36595 at a 1/1300 dilution (Figure 1), and normal mouse serum at a 1/20 dilution (Figure 2), staining paraffin embedded human normal lymph node by immunohistochemistry. HRP-anti-mouse was used as the secondary antibody before colour development with DAB.

References for Anti-CD14 antibody [5A3B11B5] (ab36595)

ab36595 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your further questions.

I can confirm the following prices:
CD14 antibody [5A3B11B5] (ab36595) 100 ul £227.00
CD14 antibody [7] (ab49755) 200 ug £227.00
Delivery: £16.00 to United Kingdom

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"