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Anti-CD14 antibody [RPA-M1] (FITC) (ab86896)

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Overview

  • Product nameAnti-CD14 antibody [RPA-M1] (FITC)See all CD14 primary antibodies ...
  • Description
    Mouse monoclonal [RPA-M1] to CD14 (FITC)
  • ConjugationFITC
  • Tested applicationsFlow Cyt more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive controlHuman monocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab86896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt
  • Application notesFlow Cyt (Cell Surface): Use 10µl for 106 cells.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionCooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
    • Tissue specificityExpressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
    • Sequence similaritiesContains 11 LRR (leucine-rich) repeats.
    • Post-translational
      modifications      N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
    • Cellular localizationCell membrane.

      • Target information above from: UniProt accession P08571 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          CD 14 antibodyCD14 antibodyCD14 antibody
          CD14 antigen antibodyCD14 molecule antibodyCD14_HUMAN antibodyLPS-R antibodyMo2 antibodyMonocyte differentiation antigen CD14 antibodyMonocyte differentiation antigen CD14, membrane-bound form antibodyMyeloid cell specific leucine rich glycoprotein antibodyMyeloid cell-specific leucine-rich glycoprotein antibody
        see all

      Anti-CD14 antibody [RPA-M1] (FITC) images

      • Flow Cytometry - CD14 antibody [RPA-M1] (FITC) (ab86896)
        Flow Cytometry - CD14 antibody [RPA-M1] (FITC) (ab86896)
        ab86896 at 10µl/106 cells staining CD14 in human monocytes by flow cytometry.
        Shaded histogram represents cells without antibody; green represents isotype control; red represents ab86896.

      References for Anti-CD14 antibody [RPA-M1] (FITC) (ab86896)

      ab86896 has not yet been referenced specifically in any publications.

      Publishing research using ab86896 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab86896 Submit a Question for ab86896

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      Hi,   Can you kindly confirmed the concentration for CD14-FITC (ab86896)? Concentration on datasheet states “ 100ug at mg/mL”…   Your soonest reply is highly appreciated as we are submitting for a tender.   Best regards,  

      Thank your for your inquiry. I can confirm that this product has a concentration of 25ug/ml. I wish you good luck for your tender.

                          Hi,   Our customer would like to know if the following antibodies are of IgG1, kappa  or lambda light chains (Information on datasheet only stated Isotypes IgG1/ IgM/ IgG2a)?   Item                                      Format          ...

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      Thank you for contacting us. In general, the immunoglobulin class (IgG1, IgG2a, IgGM, etc.) and light chain sub-class (kappa or lambda) is listed on the online datasheet. When the light chain is not listed, then the light chain sub-class has not been ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"