CD147 293T Transfected Lysate - (positive control) (ab94097)

Overview

  • Product nameCD147 293T Transfected Lysate - (positive control)
  • General notesab94097 is a 293T cell transfected lysate in which Human CD147 has been transiently over-expressed using a pCMV-CD147 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWBmore details

Properties

  • BackgroundFunction: Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. Tissue specificity: Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. PTM: N-glycosylated.

Applications

Our Abpromise guarantee covers the use of ab94097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • CD147 293T Transfected Lysate - (positive control) images

    • ab94097 at 15µg/lane on an SDS-PAGE gel.
    • All lanes : Anti-CD147 antibody (ab88530) at 1/500 dilution

      Lane 1 : CD147 293T Transfected Lysate - (positive control) (ab94097)
      Lane 2 : 293T Non transfected Lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for CD147 293T Transfected Lysate - (positive control) (ab94097)

    ab94097 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"