CD147 antibody [AB1843] (ab49493)

Product Name 

CD147 antibody [AB1843]

Product type 

Primary antibodies

Description 

Mouse monoclonal [AB1843] to CD147

Immunogen 

Prokaryotic recombinant fusion protein corresponding to a 147 amino acid region of the extracellular domain of the human CD147 molecule

Reacts with 
(species key)

Hu

Tested applications 
(see key)

IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/20 - 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

WB: 1/100. Predicted molecular weight: 42 kDa.

Is unsuitable for IHC-Fr.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human skin (IHC), SAOS-2 cell line (WB).

Cellular localization 

Type I membrane protein

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Sensory System >> Visual system
Immunology >> Cell Type Markers >> CD >> Endothelial Cells

Relevance 

CD147 plays a pivotal role in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. CD147 also seems to be a receptor for oligomannosidic glycans. In vitro, it promotes outgrowth of astrocytic processes. CD147 forms homooligomers in a cis-dependent manner on the plasma membrane. It forms a complex with MMP1 at the tumor cell surface. CD147 interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. CD147 also interacts with ATP1B2, MAG and L1CAM.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

AB1843

Myeloma 

P3-NS1/1-Ag4-1

Isotype 

IgG1

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: Tissue culture supernatant

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

See below for CD147 antibody [AB1843] images, references, products related to ab49493 and other tools.

CD147 antibody [AB1843] images:

References for CD147 antibody [AB1843] (ab49493)

  This product has been used in:

Kuang YH et al. RNA interference targeting the CD147 induces apoptosis of multi-drug resistant cancer cells related to XIAP depletion. Cancer Lett 276:189-95 (2009). WB; Human. PubMed: 19097686

If you publish research using ab49493 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding CD147 antibody [AB1843]

Customer FAQs regarding CD147 antibody [AB1843]

Protocols for CD147 antibody [AB1843]

Price and availability of products related to CD147 antibody [AB1843]

CD147 antibody [AB1843] - related products: