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Anti-CD147 antibody [MEM-M6/1] (ab666)

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Overview

Product name

Anti-CD147 antibody [MEM-M6/1]
See all CD147 products (31) ...

Description

Mouse monoclonal [MEM-M6/1] to CD147

Specificity

Human CD147 antigen. This antibody recognizes an epitope in the N-terminal Ig domain (D1). This high-affinity antibody is capable of binding to unstimulated peripheral blood T cells.

Tested applications

IHC-P, Flow Cyt, IP, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimer).

Positive control

IHC-P: human testis

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

>95% by SDS-PAGE

Purification notes

Purified from ascites by protein A-affinity chromatography.

Clonality

Monoclonal

Clone number

MEM-M6/1

Isotype

IgG1

Research areas

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Sensory System >> Visual system
Immunology >> Cell Type Markers >> CD >> Endothelial Cells

  • Flow Cytometry - CD147 antibody [MEM-M6/1] (ab666)Flow Cytometry - CD147 antibody [MEM-M6/1] (ab666) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.

Tissue specificity

Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.

Sequence similarities

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Post-translational
modifications

N-glycosylated.

Cellular localization

Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Target information above from: UniProt accessionP35613 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 5A11 antigen antibody
  • 5F7 antibody
  • BASI_HUMAN antibody
  • Basigin (Ok blood group) antibody
  • Basigin antibody
  • Blood brain barrier HT7 antigen antibody
  • Blood-brain barrier HT7 antigen antibody
  • BSG antibody
  • CD 147 antibody
  • CD147 antibody
  • CD147 antigen antibody
  • Collagenase stimulatory factor antibody
  • Emmprin antibody
  • Extracellular matrix metalloproteinase inducer antibody
  • Leukocyte activation antigen M6 antibody
  • M 6 antibody
  • M6 antibody
  • M6 leukocyte activation antigen antibody
  • neurothelin antibody
  • OK antibody
  • OK blood group antibody
  • OK blood group antigen antibody
  • TCSF antibody
  • Tumor cell derived collagenase stimulatory factor antibody
  • Tumor cell-derived collagenase stimulatory factor antibody
see all

Database links

Anti-CD147 antibody [MEM-M6/1] images:

  Flow Cytometry - CD147 antibody [MEM-M6/1] (ab666)

Flow Cytometry - CD147 antibody [MEM-M6/1] (ab666)

Overlay histogram showing peripheral blood lymphocytes stained with ab666 (red line). The cells were incubated with the antibody (ab666, 1µg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.

References for Anti-CD147 antibody [MEM-M6/1] (ab666)

This product has been referenced in:

  • Wu LSet al. A miRNA-492 binding-site polymorphism in BSG (basigin) confers risk to psoriasis in Central South Chinese population. Hum Genet 130:749-57 (2011). WB; Human.Read more (PubMed: 21655935) »
  • Nahalkova Jet al. CD147, a gamma-secretase associated protein is upregulated in Alzheimer's disease brain and its cellular trafficking is affected by presenilin-2. Neurochem Int 56:67-76 (2010).Read more (PubMed: 19751784) »

See all 6 publications for this product

Publishing research using ab666? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"