CD147 antibody [MEM-M6/1] (ab666)

Product Name 

CD147 antibody [MEM-M6/1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [MEM-M6/1] to CD147

Immunogen 

Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, C_H2-and C_H3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimer).

Reacts with 
(species key)

Hu

Specificity 

Human CD147 antigen.
This antibody recognizes an epitope in the N-terminal Ig domain (D1).
This high-affinity antibody is capable of binding to unstimulated peripheral blood T cells.

Tested applications 
(see key)

Flow Cyt, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependant concentration.
IP: Use at an assay dependant concentration.
WB: Use at a concentration of 1 µg/ml. Use under non-reducing conditions.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Type I membrane protein

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Sensory System >> Visual system
Immunology >> Cell Type Markers >> CD >> Endothelial Cells

Relevance 

CD147 plays a pivotal role in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. CD147 also seems to be a receptor for oligomannosidic glycans. In vitro, it promotes outgrowth of astrocytic processes. CD147 forms homooligomers in a cis-dependent manner on the plasma membrane. It forms a complex with MMP1 at the tumor cell surface. CD147 interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. CD147 also interacts with ATP1B2, MAG and L1CAM.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

MEM-M6/1

Isotype 

IgG1

Purity 

>95% by SDS-PAGE

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from ascites by protein A-affinity chromatography.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for CD147 antibody [MEM-M6/1] (ab666)

  This product has been used in: (two most recent references)

Kato N et al. The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion. J Am Soc Nephrol 20:1565-76 (2009). PubMed: 19443639

Till A et al. A role for membrane-bound CD147 in NOD2-mediated recognition of bacterial cytoinvasion. J Cell Sci 121:487-95 (2008). WB; Human. PubMed: 18256385

See all 4 publication references for this product.


If you publish research using ab666 please let us know so that we can cite the reference on this datasheet.

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