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Products:Immunology >> Cell Type Markers >> CD >> Endothelial Cells
Overview
Product name
Anti-CD147 antibody [MEM-M6/2]
See all CD147 products (30) ...
Description
Mouse monoclonal [MEM-M6/2] to CD147
Tested applications
Flow Cyt, IHC-Pmore details
Cross reactivity
Reacts with
Human, Pig
Immunogen
A soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the entire extracellular region of CD147 fused to the DNA coding for the hinge region, CH2 and CH3 domain of human IgG1.
Epitope
This antibody recognises extracellular Ig domain D1 of CD147 antigen.
Positive control
Testis
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
> 95% (by SDS-PAGE).
Clonality
Monoclonal
Clone number
MEM-M6/2
Isotype
IgG2b
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Sensory System >> Visual system
Immunology >> Cell Type Markers >> CD >> Endothelial Cells
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use a concentration of 4 µg/ml
IHC-P: Use a concentration of 10 µg/ml
Target
Function
Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
Tissue specificity
Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Post-translational
modifications
N-glycosylated.
Cellular localization
Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP35613
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5A11 antigen antibody
- 5F7 antibody
- 5F7 antibody
see all
Database links
- Entrez Gene: 682 Human
- Omim: 109480 Human
- SwissProt: P35613 Human
- Unigene: 501293 Human
References for Anti-CD147 antibody [MEM-M6/2] (ab21903)
ab21903 has not yet been referenced specifically in any publications.
Publishing research using ab21903? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"