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Products:Immunology >> Cell Type Markers >> CD >> Endothelial Cells
Overview
Product name
Anti-CD147 antibody
See all CD147 products (31) ...
Description
Goat polyclonal to CD147
Tested applications
IHC-P, WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant extracellular domain (Human), expressed in mouse myeloma NSO cells.
General notes
Do not store in frost-free freezer.
Endotoxin level is < 15 ng/mg antibody as determined by the LAL (Limulus amebocyte lysate) method.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Sensory System >> Visual system
Immunology >> Cell Type Markers >> CD >> Endothelial Cells
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
WB
ELISA
Application notes
IHC-P: 1/25.
ELISA: Use at a concentration of 0.5 - 1 µg/ml. The detection limit for recombinant mouse EMMPRIN is approximately 0.2 ng/well.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for mouse EMMPRIN is approximately 1 ng/lane under non-reducing and reducing conditions. Detects a band of approximately 58 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
Tissue specificity
Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Post-translational
modifications
N-glycosylated.
Cellular localization
Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP35613
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5A11 antigen antibody
- 5F7 antibody
- 5F7 antibody
see all
Database links
- Entrez Gene: 682 Human
- Entrez Gene: 12215 Mouse
- Omim: 109480 Human
- SwissProt: P35613 Human
- SwissProt: P18572 Mouse
- Unigene: 501293 Human
- Unigene: 431532 Mouse
- Unigene: 726 Mouse
References for Anti-CD147 antibody (ab11572)
This product has been referenced in:
- Hernández Iet al. Novel alternatively spliced ADAM8 isoforms contribute to the aggressive bone metastatic phenotype of lung cancer. Oncogene 29:3758-69 (2010).Read more (PubMed: 20453887) »
- Kirwan RPet al. Influence of cyclical mechanical strain on extracellular matrix gene expression in human lamina cribrosa cells in vitro. Mol Vis 11:798-810 (2005).Read more (PubMed: 16205625) »
See all 2 publications for this product
Publishing research using ab11572? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"