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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab64616? Please let us know so that we can cite the reference in this datasheet
ab64616 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - CD147 antibody (ab64616)
All lanes : Anti-CD147 antibody (ab64616) at 1 µg/ml
Lane 1 : HeLa Whole Cell Lysate - Hydroxyurea Treated (48hr, 2uM)
Lane 2 : HeLa Whole Cell Lysate - Bleomycin Treated (20U/ml)
Lane 3 : HeLa Whole Cell Lysate - Staurosporine Treated (48hr, 500nM)
Lane 4 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 5 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 7 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 42 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.
CD147 contains known glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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