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Western blot - CD147 antibody (ab64616)
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Product Name
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CD147 antibody
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See all CD147 antibodies (25)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CD147
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human CD147. (Peptide available as ab71911.)
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Orngt
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 42 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following Lysates:
HeLa Whole Cell - Hydroxyurea Treated (48hr, 2uM), HeLa Whole Cell - Bleomycin Treated (20U/ml), HeLa Whole Cell - Staurosporine Treated (48hr, 500nM), Y79 Whole Cell, Mouse Skeletal Muscle Tissue, HeLa Whole Cell, SK N SH Whole Cell
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Cellular localization
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Type I membrane protein
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Sensory System >> Visual system
Immunology >> Cell Type Markers >> CD >> Endothelial Cells
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Relevance
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CD147 plays a pivotal role in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. CD147 also seems to be a receptor for oligomannosidic glycans. In vitro, it promotes outgrowth of astrocytic processes. CD147 forms homooligomers in a cis-dependent manner on the plasma membrane. It forms a complex with MMP1 at the tumor cell surface. CD147 interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. CD147 also interacts with ATP1B2, MAG and L1CAM.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.300 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD147 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD147 antibody images, references, products related to ab64616 and other tools.
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CD147 antibody images:
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 Western blot - CD147 antibody (ab64616)
All lanes : CD147 antibody (ab64616) at 1 µg/ml
Lane 1 : HeLa Whole Cell Lysate - Hydroxyurea Treated (48hr, 2uM)
Lane 2 : HeLa Whole Cell Lysate - Bleomycin Treated (20U/ml)
Lane 3 : HeLa Whole Cell Lysate - Staurosporine Treated (48hr, 500nM)
Lane 4 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 5 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 7 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 42 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.
CD147 contains known glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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Search PubMed (MEDLINE) for references to CD147
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CD147 antibody - more information
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Customer reviews (feedback) regarding CD147 antibody |
Customer FAQs regarding CD147 antibody |
Protocols for CD147 antibody |
Price and availability of products related to CD147 antibody |
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CD147 antibody - related products:
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CD147 antibody - other tools:
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Contact Abcam with a Technical Enquiry about CD147 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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