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Products:Immunology >> Cell Type Markers >> CD >> Endothelial Cells
Overview
Product name
Anti-CD147 antibody
See all CD147 products (31) ...
Description
Rabbit polyclonal to CD147
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Orangutan
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human CD147.
(Peptide available as ab71911.)
Positive control
This antibody gave a positive signal in the following Lysates: HeLa Whole Cell - Hydroxyurea Treated (48hr, 2uM), HeLa Whole Cell - Bleomycin Treated (20U/ml), HeLa Whole Cell - Staurosporine Treated (48hr, 500nM), Y79 Whole Cell, Mouse Skeletal Muscle Tissue, HeLa Whole Cell, SK N SH Whole Cell
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Sensory System >> Visual system
Immunology >> Cell Type Markers >> CD >> Endothelial Cells
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Western blot - CD147 antibody (ab64616)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab64616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 60 kDa (predicted molecular weight: 42 kDa).
Target
Function
Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
Tissue specificity
Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Post-translational
modifications
N-glycosylated.
Cellular localization
Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP35613
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5A11 antigen antibody
- 5F7 antibody
- BASI_HUMAN antibody
see all
Database links
- Entrez Gene: 682 Human
- Entrez Gene: 12215 Mouse
- Omim: 109480 Human
- SwissProt: P35613 Human
- SwissProt: P18572 Mouse
- Unigene: 501293 Human
- Unigene: 431532 Mouse
- Unigene: 726 Mouse
Anti-CD147 antibody images:
Western blot - CD147 antibody (ab64616)
All lanes : Anti-CD147 antibody (ab64616) at 1 µg/ml
Lane 1 : HeLa Whole Cell Lysate - Hydroxyurea Treated (48hr, 2uM)
Lane 2 : HeLa Whole Cell Lysate - Bleomycin Treated (20U/ml)
Lane 3 : HeLa Whole Cell Lysate - Staurosporine Treated (48hr, 500nM)
Lane 4 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 5 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 7 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 42 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.
CD147 contains known glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
References for Anti-CD147 antibody (ab64616)
ab64616 has not yet been referenced specifically in any publications.
Publishing research using ab64616? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"