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Overview

  • Product nameAnti-CD147 antibodySee all CD147 primary antibodies ...
  • Description
    Rabbit polyclonal to CD147
  • Tested applicationsWB, ELISA, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from C terminus of human CD147

  • Positive controlHuman brain tissue, extracts from HUVEC cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab70062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 42 kDa).
ELISA ELISA: 1/10000.
IHC-P IHC-P: 1/50 - 1/100.

Target

  • FunctionPlays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
  • Tissue specificityPresent only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
  • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications    N-glycosylated.
  • Cellular localizationCell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

    • Target information above from: UniProt accession P35613 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      5A11 antigen antibody5F7 antibodyBASI_HUMAN antibody
      Basigin (Ok blood group) antibodyBasigin antibodyBlood brain barrier HT7 antigen antibodyBSG antibodyCD 147 antibodyCD147 antibodyCD147 antigen antibodyCollagenase stimulatory factor antibodyEmmprin antibodyExtracellular matrix metalloproteinase inducer antibodyLeukocyte activation antigen M6 antibodyM 6 antibodyM6 antibodyM6 antigen antibodyM6 leukocyte activation antigen antibodyNeurothelin antibodyOK antibodyOK blood group antibodyOK blood group antigen antibodyTCSF antibodyTumor cell derived collagenase stimulatory factor antibodyTumor cell-derived collagenase stimulatory factor antibody
    see all

Anti-CD147 antibody images

  • Western blot - CD147 antibody (ab70062)
    Western blot - CD147 antibody (ab70062)
    All lanes : Anti-CD147 antibody (ab70062) at 1/500 dilution

    Lane 1 : extracts from HUVEC cells
    Lane 2 : extracts from HUVEC cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 42 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD147 antibody (ab70062)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD147 antibody (ab70062)
    Human brain tissue with ab70062 at 1/50 dilution, with and without the immunising peptide.

References for Anti-CD147 antibody (ab70062)

This product has been referenced in:
  • Winkler IG  et al. Vascular niche E-selectin regulates hematopoietic stem cell dormancy, self renewal and chemoresistance. Nat Med 18:1651-7 (2012). WB ; Mouse . Read more (PubMed: 23086476) »

See 1 Publication for this product

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