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Products:Immunology >> Cell Type Markers >> CD >> Endothelial Cells
Overview
Product name
Anti-CD147 antibody
See all CD147 products (31) ...
Description
Rabbit polyclonal to CD147
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide derived from C terminus of human CD147
Positive control
Human brain tissue, extracts from HUVEC cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Sensory System >> Visual system
Immunology >> Cell Type Markers >> CD >> Endothelial Cells
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 49 kDa (predicted molecular weight: 42 kDa).
ELISA
ELISA: 1/10000ELISA: 1/10000
IHC-P: 1/50 - 1/100.
Target
Function
Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
Tissue specificity
Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Post-translational
modifications
N-glycosylated.
Cellular localization
Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP35613
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5A11 antigen antibody
- 5F7 antibody
- BASI_HUMAN antibody
see all
Database links
- Entrez Gene: 682 Human
- Entrez Gene: 12215 Mouse
- Entrez Gene: 25246 Rat
- Omim: 109480 Human
- SwissProt: P35613 Human
- SwissProt: P18572 Mouse
- SwissProt: P26453 Rat
- Unigene: 501293 Human
see all
Anti-CD147 antibody images:
Western blot - CD147 antibody (ab70062)
All lanes : Anti-CD147 antibody (ab70062) at 1/500 dilution
Lane 1 : extracts from HUVEC cells
Lane 2 : extracts from HUVEC cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 42 kDa
Observed band size : 49 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD147 antibody (ab70062)
Human brain tissue with ab70062 at 1/50 dilution, with and without the immunising peptide.
References for Anti-CD147 antibody (ab70062)
ab70062 has not yet been referenced specifically in any publications.
Publishing research using ab70062? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"