 image](#CD15-Primary-antibodies-ab6414-1.jpg)
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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-CD15 antibody [MC-1]
See all CD15 products (30) ...
Description
Mouse monoclonal [MC-1] to CD15
Specificity
Detects the CD15 antigen (Mw 150kDa, 95kDa) found on neutrophils, eosinophils and moncytes. Reed Sternberg cells in Hodgins lymphoma are positive with this antibody.
Tested applications
IHC-P, Flow Cytmore details
Cross reactivity
Reacts with
Human
General notes
This antibody has the ability to fix complement.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Purified IgM
Clonality
Monoclonal
Clone number
MC-1
Isotype
IgM
Research areas
Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Cancer >> Tumor immunology >> CD markers
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative
Immunology >> Cell Type Markers >> CD >> Adhesion
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CD15 antibody [MC-1](ab6414)
 image](/ps/datasheet/images/6/ab6414/CD15-Primary-antibodies-ab6414-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab6414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
Flow Cyt
Application notes
Flow Cyt: 1/300.
IHC-P: 1/200 - 1/400.
Samples may require neuraminidase treatment to reveal the antigen.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.
Pathway
Protein modification; protein glycosylation.
Sequence similarities
Belongs to the glycosyltransferase 10 family.
Cellular localization
Golgi apparatus > Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi.
Target information above from: UniProt accessionP22083
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alpha (1,3) fucosyltransferase antibody
- Alpha 13 fucosyltransferase FucT antibody
- Alpha-(1,3)-fucosyltransferase antibody
see all
Database links
- Entrez Gene: 10690 Human
- Entrez Gene: 2526 Human
- Entrez Gene: 2527 Human
- Entrez Gene: 2528 Human
- Entrez Gene: 2529 Human
- Omim: 104230 Human
- SwissProt: P22083 Human
- SwissProt: P51993 Human
see all
Anti-CD15 antibody [MC-1] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CD15 antibody [MC-1](ab6414)
ab6414 (1µg/ml) staining CD15 in granulocytes of human myometrium.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-CD15 antibody [MC-1] (ab6414)
ab6414 has not yet been referenced specifically in any publications.
Publishing research using ab6414? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"