CD153 Mouse ELISA Kit (ab100672)
- Product nameCD153 Mouse ELISA KitSee all CD153 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 0.5 pg/ml
- Range0.41 pg/ml - 100 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 101.34 91% - 110% Serum 96.78 87% - 108% Plasma 95.79 85% - 107%
- Species reactivityReacts with: Mouse
- Product overview
ab100672 CD153 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CD153 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse CD153 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD153 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CD153 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD153 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium) diluent. 1 x 15ml CD153 Microplate (12 strips x 8 wells) coated with anti-mouse CD153 1 unit Detection Antibody CD153: biotinylated anti-mouse CD153 2 vials HRP-Streptavidin Concentrate 100x concentrated HRP-conjugated streptavidin 1 x 200µl Mouse CD153 Standard (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- CD153 antigenCD30 antigen ligandCD30 L
- CD30 ligand CD30LCD30LGMGC138144TNFSF8Tumor necrosis factor (ligand) superfamily member 8 Tumor necrosis factor ligand superfamily member 8
Our Abpromise guarantee covers the use of ab100672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for CD153 Mouse ELISA Kit (ab100672)
ab100672 has not yet been referenced specifically in any publications.