Anti-CD16+CD32 antibody [93] - Low endotoxin, Azide free (ab171202)
Key features and details
- Rat monoclonal [93] to CD16+CD32 - Low endotoxin, Azide free
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG2a
Related conjugates and formulations
Overview
-
Product name
Anti-CD16+CD32 antibody [93] - Low endotoxin, Azide free
See all CD16+CD32 primary antibodies -
Description
Rat monoclonal [93] to CD16+CD32 - Low endotoxin, Azide free -
Host species
Rat -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
The details of the immunogen for this antibody are not available.
-
Positive control
- Flow cyt: Mouse splenocyte suspensions. ICC/IF: Raw264.7 cells
-
General notes
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by the LAL assay.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
93 -
Isotype
IgG2a -
Light chain type
lambda -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 10 µg/ml.
|
|
Flow Cyt |
Use 0.5µg for 105-8 cells.
(in a final volume of 100 μL). ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
---|
ICC/IF
Use a concentration of 10 µg/ml. |
Flow Cyt
Use 0.5µg for 105-8 cells. (in a final volume of 100 μL). ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
-
Relevance
Function: Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. Tissue specificity: Found on monocytes, neutrophils and eosinophil platelets. Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. PTM: Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK. -
Cellular localization
CD32: Type I membrane protein. CD16: Attached to the membrane by a GPI anchor. Exists also as a soluble receptor, produced by a proteolytic cleavage. -
Database links
- Entrez Gene: 2212 Human
- Entrez Gene: 2213 Human
- Entrez Gene: 2214 Human
- Entrez Gene: 2215 Human
- Entrez Gene: 14130 Mouse
- Entrez Gene: 14131 Mouse
- Omim: 146740 Human
- SwissProt: O75015 Human
see all -
Alternative names
- CD16a antibody
- CD16b antibody
- CD32 antibody
see all
Images
-
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab25235)
ab25235 staining CD16 + CD32 in Raw264.7 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab25235 at 10µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150165, Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
-
Flow cytometric analysis of BALB/c splenocytes with 0.25 µg Rat IgG2a K Isotype Control (open histogram) or 0.25 µg ab171202 (filled histogram) followed by Anti-Rat IgG FITC. Total viable cells were used for analysis.
Datasheets and documents
-
Datasheet download
References (0)
ab171202 has not yet been referenced specifically in any publications.