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Anti-CD18 antibody [CLB-LFA-1/1, 54] (FITC) (ab51573)

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Overview

Product name

Anti-CD18 antibody [CLB-LFA-1/1, 54] (FITC)
See all CD18 products (44) ...

Description

Mouse monoclonal [CLB-LFA-1/1, 54] to CD18 (FITC)

Conjugation

FITC

Conjugation notes

The antibody is conjugated with fluorescein isothiocyanate isomer 1 (FITC). The molecular F/P ratio is between 5 and 10.

Specificity

ab51573 is directed against the CD18 antigen (Lymphocyte Function-associated Antigen 1, integrin beta 2 chain), which is expressed on mature immunocompetent lymphocytes and their neoplastic counterparts, granulocytes and monocytes. It is absent in patients with LAD1.

Tested applications

Flow Cytmore details

Cross reactivity

Reacts with

Human

Immunogen

Peripheral blood lymphocytes of a T gamma lymphocytosis patient (Human)

Properties

Form

Liquid

Storage instructions

Store at +4°C in the dark. Do not freeze.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 1% (w/v)BSA, 20mM Tris, 150mM Sodium chloride, pH 8.0

Concentration

Concentration information loading...

Purity

Ion Exchange Chromatography

Purification notes

The antibody was purified from ascites using column chromatography

Primary antibody notes

The monoclonal antibody can be used to detect LFA1/MO1 deficiency in patients suffering from recurrent bacterial infections.

Clonality

Monoclonal

Clone number

CLB-LFA-1/1, 54

Myeloma

Sp2/0

Isotype

IgG1

Research areas

Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Mast Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> NK Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Immunology >> Cell Type Markers >> CD >> Adhesion
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta

  • Flow Cytometry - CD18 antibody [CLB-LFA-1/1, 54] (FITC) (ab51573)Flow Cytometry - CD18 antibody [CLB-LFA-1/1, 54] (FITC) (ab51573) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab51573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Flow Cyt: Use 10µl for 40µl of 107 cells/ml suspension or 20µl per 100µl whole blood.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.

Involvement in disease

Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.

Sequence similarities

Belongs to the integrin beta chain family.
Contains 1 VWFA domain.

Post-translational
modifications

Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.

Cellular localization

Membrane.

Target information above from: UniProt accessionP05107 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 95 subunit beta antibody
  • CD 18 antibody
  • CD18 antibody
  • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
  • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
  • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
  • Complement receptor C3 beta subunit antibody
  • Complement receptor C3 subunit beta antibody
  • Integrin beta 2 antibody
  • Integrin beta chain beta 2 antibody
  • Integrin beta-2 antibody
  • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
  • ITB2_HUMAN antibody
  • ITGB2 antibody
  • LAD antibody
  • LCAMB antibody
  • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
  • Leukocyte cell adhesion molecule CD18 antibody
  • LFA 1 antibody
  • LFA1 antibody
  • Lymphocyte function associated antigen 1 antibody
  • MAC 1 antibody
  • MAC1 antibody
  • MF17 antibody
  • MFI7 antibody
  • OTTHUMP00000115278 antibody
  • OTTHUMP00000115279 antibody
  • OTTHUMP00000115280 antibody
  • OTTHUMP00000115281 antibody
  • OTTHUMP00000115282 antibody
see all

Database links

Anti-CD18 antibody [CLB-LFA-1/1, 54] (FITC) images:

  Flow Cytometry - CD18 antibody [CLB-LFA-1/1, 54] (FITC) (ab51573)

Flow Cytometry - CD18 antibody [CLB-LFA-1/1, 54] (FITC) (ab51573)

Displays representative data performed on gated lymphocytes. Laser excitation is at 488 nm.

References for Anti-CD18 antibody [CLB-LFA-1/1, 54] (FITC) (ab51573)

This product has been referenced in:

  • Zang Qet al. The top of the inserted-like domain of the integrin lymphocyte function-associated antigen-1 beta subunit contacts the alpha subunit beta -propeller domain near beta-sheet 3. J Biol Chem 275:22202-12 (2000).Read more (PubMed: 10781608) »

See 1 publication for this product

Publishing research using ab51573? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"