![Flow Cytometry - Anti-CD18 antibody [CLB-LFA-1/1, 54] (ab51377) image](#CD18-Primary-antibodies-ab51377-78.jpg)
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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Overview
Product name
Anti-CD18 antibody [CLB-LFA-1/1, 54]
See all CD18 products (44) ...
Description
Mouse monoclonal [CLB-LFA-1/1, 54] to CD18
Specificity
ab51377 is directed against the CD18 antigen (Lymphocyte Function-associated Antigen-1, integrin ß2 chain), which is expressed on mature immunocompetent lymphocytes and their neoplastic counterparts, granulocytes and monocytes. It is absent in patients with LAD-1.
Tested applications
Flow Cyt, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Tissue / cell preparation: patient cells
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark.
Storage buffer
Preservative: 0.001% Thimerosal (merthiolate)
Constituents: 10mg/ml BSA, 20mM Tris, 150mM Sodium chloride, pH 8
Concentration
Concentration information loading...
Purity
Ion Exchange Chromatography
Purification notes
ab51377 was purified from ascites by ammonium sulphate precipitation and ion exchange chromatography.
Clonality
Monoclonal
Clone number
CLB-LFA-1/1, 54
Myeloma
Sp2/0
Isotype
IgG1
Research areas
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Mast Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> NK Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Immunology >> Cell Type Markers >> CD >> Adhesion
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
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Flow Cytometry - Anti-CD18 antibody [CLB-LFA-1/1, 54] (ab51377)
![Flow Cytometry - Anti-CD18 antibody [CLB-LFA-1/1, 54] (ab51377) image](/ps/datasheet/images/51/ab51377/CD18-Primary-antibodies-ab51377-78.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab51377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
Target
Function
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
Involvement in disease
Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 VWFA domain.
Post-translational
modifications
Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
Cellular localization
Membrane.
Target information above from: UniProt accessionP05107
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 95 subunit beta antibody
- CD 18 antibody
- CD18 antibody
see all
Database links
- Entrez Gene: 3689 Human
- Omim: 600065 Human
- SwissProt: P05107 Human
- Unigene: 375957 Human
Anti-CD18 antibody [CLB-LFA-1/1, 54] images:
Flow Cytometry - Anti-CD18 antibody [CLB-LFA-1/1, 54] (ab51377)
ab51377 staining CD18 in Human monocyte-derived macrophages by Flow Cytometry. Cells were cultured in PBS + 1% BSA. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA) for 1 hour at 4°C. A FITC-conjugated Goat anti-mouseIgG (1/100) secondary antibody was used.
Gating Strategy: Live Cells.
This image is courtesy of an anonymous Abreview
References for Anti-CD18 antibody [CLB-LFA-1/1, 54] (ab51377)
This product has been referenced in:
- Miedema Fet al. Immunologic studies with LFA-1- and Mo1-deficient lymphocytes from a patient with recurrent bacterial infections. J Immunol 134:3075-81 (1985).Read more (PubMed: 3156928) »
- Miedema Fet al. Both Fc receptors and lymphocyte-function-associated antigen 1 on human T gamma lymphocytes are required for antibody-dependent cellular cytotoxicity (killer cell activity). Eur J Immunol 14:518-23 (1984).Read more (PubMed: 6610556) »
See all 2 publications for this product
Publishing research using ab51377? Please let us know so that we can cite the reference in this datasheet
![Anti-CD18 antibody [CLB-LFA-1/1, 54] for Flow Cytometry in Mouse (51377)](/ps/datasheet/images/51/ab51377/CD18-Primary-antibodies-ab51377-85.jpg)
![Anti-CD18 antibody [CLB-LFA-1/1, 54] for Flow Cytometry in Human (51377)](/ps/datasheet/images/51/ab51377/CD18-Primary-antibodies-ab51377-75.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"