![Flow Cytometry - CD19 antibody [MB19-1] (Phycoerythrin) (ab93562) image](#CD19-Primary-antibodies-ab93562-1.jpg)
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Product name
Anti-CD19 antibody [MB19-1] (Phycoerythrin)
See all CD19 products (62) ...
Description
Mouse monoclonal [MB19-1] to CD19 (Phycoerythrin)
Conjugation
Phycoerythrin
Tested applications
Flow Cytmore details
Cross reactivity
Reacts with
Mouse
Immunogen
CD19+ Mouse pre-B cell line, 300.19
Positive control
BALB/c splenocytes and Mouse splenocytes
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark. Do not freeze.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS, 500mM Sodium chloride, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
MB19-1
Isotype
IgA
Light chain type
kappa
Research areas
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative
Immunology >> Adaptive Immunity >> B Cells >> CD
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Flow Cytometry - CD19 antibody [MB19-1] (Phycoerythrin) (ab93562)
![Flow Cytometry - CD19 antibody [MB19-1] (Phycoerythrin) (ab93562) image](/ps/datasheet/images/93/ab93562/CD19-Primary-antibodies-ab93562-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab93562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
Flow Cyt
Application notes
Flow Cyt: Use 0.5 µg for 105 to 108 cells in a final volume of 100 µl.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Involvement in disease
Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Post-translational
modifications
Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
Cellular localization
Membrane.
Target information above from: UniProt accessionP15391
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Antibody deficiency due to defect in CD19, included antibody
- AW495831 antibody
- B lymphocyte antigen CD19 antibody
see all
Database links
- Entrez Gene: 12478 Mouse
- SwissProt: P25918 Mouse
- Unigene: 4360 Mouse
Anti-CD19 antibody [MB19-1] (Phycoerythrin) images:
Flow Cytometry - CD19 antibody [MB19-1] (Phycoerythrin) (ab93562)
Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or ab93562 (colored histogram). Cells in the lymphocyte gate were used for analysis.
References for Anti-CD19 antibody [MB19-1] (Phycoerythrin) (ab93562)
ab93562 has not yet been referenced specifically in any publications.
Publishing research using ab93562? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"