![Western blot - Anti-CD1a antibody [EP3091] (ab76531) image](#CD1a-Primary-antibodies-ab76531-2.jpg)
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Products:Immunology >> Adaptive Immunity >> T Cells >> CD
Overview
Product name
Anti-CD1a antibody [EP3091]
See all CD1a products (22) ...
Description
Rabbit monoclonal [EP3091] to CD1a
Tested applications
WB, ICCmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
Synthetic peptide corresponding to residues on human CD1a was used as an immunogen.
Positive control
Mouse thymus, MOLT4, K562 and fetal thymus lysates
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP3091
Isotype
IgG
Research areas
Immunology >> Adaptive Immunity >> T Cells >> CD
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Western blot - Anti-CD1a antibody [EP3091] (ab76531)
![Western blot - Anti-CD1a antibody [EP3091] (ab76531) image](/ps/datasheet/images/76/ab76531/CD1a-Primary-antibodies-ab76531-2.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/10000.Predicted molecular weight: 37 kDa.
ICC
ICC: 1/100 - 1/250.ICC: 1/100 - 1/250.
Application notes
Is unsuitable for ,Flow Cyt,IHC-P or IP.
Target
Function
Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
Tissue specificity
Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Cellular localization
Cell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes. Localizes to cell surface lipid rafts.
Target information above from: UniProt accessionP06126
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 1a antibody
- CD1 antibody
- CD1a antibody
see all
Database links
- Entrez Gene: 909 Human
- Omim: 188370 Human
- SwissProt: P06126 Human
- Unigene: 1309 Human
Anti-CD1a antibody [EP3091] images:
Western blot - Anti-CD1a antibody [EP3091] (ab76531)
All lanes : Anti-CD1a antibody [EP3091] (ab76531) at 1/5000 dilution
Lane 1 : MOLT4 lysate
Lane 2 : K562 lysate
Lane 3 : Fetal thymus lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 37 kDa
Observed band size : 47 kDa (why is the actual band size different from the predicted?)
References for Anti-CD1a antibody [EP3091] (ab76531)
ab76531 has not yet been referenced specifically in any publications.
Publishing research using ab76531? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"