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Western blot - CD1a antibody [EP3091] (ab76531)
![Western blot - CD1a antibody [EP3091] (ab76531)](/ps/datasheet/images/cd1.jpg)
(enlarge)
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Product Name
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CD1a antibody [EP3091]
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See all CD1a antibodies (16)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EP3091] to CD1a
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Immunogen
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Synthetic peptide corresponding to residues on human CD1a was used as an immunogen.
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Reacts with
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Hu, Ms
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Does not react with
(species key)
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Rat
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Tested applications
(see key)
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ICC, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: 1/100 - 1/250.
WB: 1/5000 - 1/200000. Detects a band of approximately 50 kDa (predicted molecular weight: 37 kDa).
Is unsuitable for Flow Cyt, IHC-P or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse thymus, MOLT4, K562 and fetal thymus lysates
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Cellular localization
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Cell Membrane; type I membrane protein.
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Research areas
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Immunology >> Adaptive Immunity >> T Cells >> CD
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Relevance
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CD1a is a non polymorphic MHC Class 1 related cell surface glycoprotein, expressed in association with Beta 2 microglobulin. CD1a is expressed by cortical thymocytes, Langerhan's cells and by interdigitating cells. CD1a is also expressed by some malignancies of T cell lineage and in histiocytosis X.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EP3091
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD1a antibody [EP3091] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD1a antibody [EP3091] images, references, products related to ab76531 and other tools.
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CD1a antibody [EP3091] images:
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 Western blot - CD1a antibody [EP3091] (ab76531)
Lanes 1 - 3 : CD1a antibody [EP3091] (ab76531) at 1/5000 dilution
Lane 4 : CD1a antibody [EP3091] (ab76531) at 1/200000 dilution
Lane 1 : Mouse thymus lysate
Lane 2 : MOLT4 lysate
Lane 3 : K562 lysate
Lane 4 : Fetal thymus lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 37 kDa
Observed band size : 47 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to CD1a
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CD1a antibody [EP3091] - more information
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Customer reviews (feedback) regarding CD1a antibody [EP3091] |
Customer FAQs regarding CD1a antibody [EP3091] |
Protocols for CD1a antibody [EP3091] |
Price and availability of products related to CD1a antibody [EP3091] |
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CD1a antibody [EP3091] - related products:
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CD1a antibody [EP3091] - other tools:
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Contact Abcam with a Technical Enquiry about CD1a antibody [EP3091] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - CD1a antibody [EP3091] (ab76531)](/index.html?datasheet=76531#cd1.jpg)
